I-Chymotrypsinogen A CAS: 9035-75-0 I-crystaline powder emhlophe
| Inombolo Yekhathalogu | XD90376 |
| Igama lemveliso | Chymotrypsinogen A |
| CAS | 9035-75-0 |
| Ifomula yeemolekyuli | I-C47H58FeN4O4S2 |
| Ubunzima beMolekyuli | 862.96 |
| Iinkcukacha zokuGcina | -20°C |
Ukucaciswa kweMveliso
| Isivavanyi | 99% |
| Imbonakalo | Umgubo wekristale omhlophe |
I-Adsorption of proteins to solid-fluid interfaces idla ngokufunyanwa ngamandla ukukhuthaza ukubunjwa kwe-soluble aggregates kunye namasuntswana amakhulu, abonakalayo, abonakalayo, kodwa amanqanaba aphambili kule nkqubo ahlala enzima ukuphonononga ngokuthe ngqo.Ukuhlanganiswa kwe-adsorption kwi-interfaces yamanzi-silicon oxide (i-SiOx), efana nemigangatho yeglasi ye-hydrated, ibonakaliswe njengomsebenzi we-pH kunye namandla e-ionic ye-alpha-chymotrypsinogen (aCgn) kunye ne-antibody monoclonal (IgG1).Iseli equkuqelayo ivumele i-neutron reflectivity yeprotein layers adsorbed ukucoca umphezulu we-SiOx, kunye nasemva kwamanyathelo "okuhlanjwa" alandelelanayo.Ii-aggregates zifunyenwe kwisisombululo emva kokuba "zihlanjwe" ngobunono umphezulu zibonakaliswe ngokusasazwa kwe-neutron, i-microscopy, kunye ne-fluorescence spectroscopy.Iimolekyuli ze-IgG1 ezijolise ikakhulu "flat" kumphezulu we-SiOx, kunye nomgangatho weprotheyini osisiseko ohlulwe ukuya kuthi ga kwinqanaba elincinane, ngelixa i-diffuse overlayer yasulwa ngokulula.Iimolekyuli ze-aCgn zazixhathisa ekudibaneni xa zibonakala ukuba zityhilwe kujongano, kodwa ngenye indlela zisuswe ngokulula.Kwiimeko apho ukubophelela okunamandla kwenzeka, iprotheni eyenza i-desorb yayingumxube we-monomer kunye neemali ezincinci ze-HMW aggregates (ye-aCgn) okanye amasuntswana angabonakaliyo (kwi-IgG1).Utshintsho kwi-adsorption kunye / okanye ukutyhileka nge-pH lubonise ukuba ukusebenzisana kwe-electrostatic kwakubalulekile kuzo zonke iimeko.
