dUMP,2′-Deoxyuridine 5′-monophosphate disodium ityuwa CAS:42155-08-8
| Inombolo Yekhathalogu | XD90546 |
| Igama lemveliso | Ukulahla,2'-Deoxyuridine 5'-monophosphate disodium ityuwa |
| CAS | 42155-08-8 |
| Ifomula yeemolekyuli | I-C9H11N2Na2O8P |
| Ubunzima beMolekyuli | 352.15 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 29349990 |
Ukucaciswa kweMveliso
| Isivavanyi | 99% |
Ukulinganisa ngokuthe ngqo i-metabolites ezininzi ze-5-fluorouracil (5-FU) kunye ne-endogenous monophosphate nucleotides, siphuhlise indlela yoqobo esekwe kwi-liquid chromatography-tandem mass spectrometry (LC-MS/MS).Olu vavanyo luvumele ukuzimisela: (i) ukuveliswa kwe-intracellular ye-5-fluoro-2'-deoxyuridine-5′-monophosphate (5-FdUMP) ukusuka kwi-5-FU okanye i-5-fluoro-2′-deoxyuridine (5-FdUrd) , (ii) impembelelo ye-5-FdUMP yoxinaniso kwi-intracellular 2′-deoxyuridine-5′-monophosphate (dUMP)/thymidine-5′-monophosphate (TMP) ratio, kunye (iii) nobungakanani bemfihlo ye-5-FdUMP kunye I-5-FU evela kwiiseli ezikhuliswe ngabantu ngabathuthi be-ABC.Ngaphantsi kweemeko zethu zovavanyo, iiseli zifakwe kwi-5-FU okanye i-5-FUrd.Emva koko, iiprotheyini zeselula zagqitywa yi-methanol.Le nkqubo ibonelele ukubuyiswa okuphezulu kokutsalwa.Ukongeza, ukulinganisa i-5-FU kunye ne-5-FdUMP secretion ukusuka kwiiseli, senze ubungakanani bezi molekyuli kwinkcubeko medium.I-Media mhlawumbi ifakwe ngokuthe ngqo (i-5-FU) okanye iphantsi kwesigaba esiqinileyo sokutsalwa kusetyenziswa i-Oasis Wax extraction cartridge (5-FdUMP).Ukwahlulwa kwe-analytes kwenziwa kwikholamu ye-dC18 Atlantis 3.5 μm, (100 mm × 2.1 mm id) enemowudi ye-isocratic usebenzisa i-ammonium formate buffer/methanol/water (5/5/90, v/v) njengesigaba esihambayo.Ixesha lokugijima alidlulanga i-6.2 min.Abahlalutyi benziwe i-ionized kwi-interface ye-electrospray phantsi kwemodi ye-ion embi.Siqinisekise indlela phezu koluhlu lwe-2.5-150 ng mL -1 ngokwemixube.Ukwahluka kwe-Intra- kunye no-inter-assay bekungaphantsi kwe-10% kwiintsuku ezisixhenxe.Zonke iikhompawundi bezizinzile kwiiseli okanye kwisiphakathi senkcubeko xa iisampulu zigcinwe ku-−20 °C ubuncinci iiveki ezimbini, nasemva kwemijikelo emithathu yokunyibilika komkhenkce.Akukho siphumo sematrix sabonwa kuzo zombini imidiya.
