I-6-Chloropurine CAS: 87-42-3 Umgubo ophuzi okhanyayo
| Inombolo Yekhathalogu | XD90547 |
| Igama lemveliso | 6-Chloropurine |
| CAS | 87-42-3 |
| Ifomula yeemolekyuli | C5H3ClN4 |
| Ubunzima beMolekyuli | 154.557 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 2933990090 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo otyheli okhanyayo |
| Isivavanyi | 99% |
Kutshanje siye saxela ukuba i-pyrene nucleotide ifakwe ngokukhethekileyo ngokuchasene nesiza se-abasic, i-3'-T ye-thymine dimer, kunye nezona ziseko ezingonakaliswanga yiyeast DNA polymerase eta (pol eta).Ngenxa yokuba i-pyrene i-molecule ye-nonpolar engenakho i-H-bonding, ukuphumelela okuphezulu ngokungaqhelekanga kokufakwa kwe-dPMP kuchazwe kwikhono layo eliphezulu lokubeka isiseko, kwaye kugxininise ukubaluleka kokubaluleka kwesiseko ekukhetheni i-nucleotides nge-pol eta.Ukuphanda indima ye-H-bonding kunye ne-base pair geometry ekukhetheni i-nucleotides yi-pol eta, sinqume ukufakwa kwe-nucleotides ye-base-modified nucleotides i-2,6-diaminopurine, i-2-aminopurine, i-6-chloropurine, kunye ne-inosine eya kuthi. yenza inani elahlukileyo le-H-bonds kunye nesiseko setemplate ngokuxhomekeke kwi-geometry yesiseko.I-Watson-Crick base pairing ibonakala idlala indima ebalulekileyo ekukhetheni i-nucleotide analogues yokufakelwa ngokuchasene ne-C kunye ne-T njengoko kubonakaliswe kukuhla kobuchule bokufakelwa okuhambelanayo kunye nokuncipha kwenani le-Watson-Crick H-bond kunye nokwanda kwe. inani lonxibelelwano lomxhasi kunye nommkeli-wamkelayo.Ukukhethwa kokufakwa kwe-nucleotide kukhulu ngokuchasene ne-5'-T kune-3'-T ye-thymine dimer, ngokuhambelana nomsebenzi wangaphambili obonisa ukuba i-5'-T ibanjwe ngokungqongqo kune-3'-T.Ngaphaya koko, ukufakela u-A ochaseneyo no-Ts zombini we-dimer kubonakala ngathi ulawulwa yi-Watson-Crick base pairing hayi nge-Hoogsteen base pairing esekwe kukufaneka okuphantse kufane kufakelo lwe-A kunye ne-7-deaza-A, eyokugqibela engena-H- amandla okudibanisa ku-N7.Ubuchule obunxulumeneyo bokufakelwa kwee-nucleotides ezinokuthi zenze izibini zesiseko se-Watson-Crick zihambelana nezo zeqhekeza le-Klenow, ngelixa iqhekeza le-Klenow licalula kakhulu ngokuchasene nokungafaniyo, ngokuhambelana nokukhetha kwayo okukhulu.Ezi ziphumo zigxininisa ukubaluleka kwe-H-bonding kunye ne-Watson-Crick base pair geometry ekukhethweni kwee-nucleotides zombini i-pol eta kunye neqhekeza le-Klen Ow, kunye nendima encinci yokukhetha imilo ekufakweni yi-pol eta ngenxa yokuvuleka kwayo ngakumbi nangaphantsi. indawo esebenzayo ethintelweyo.
