Iimveliso

Ukuze kucaciswe inkqubo yokuqala ye-dentin mineralization, i-inhibitory effect ye-1-hydroxyethylidene-1,1-bisphosphonate (HEBP) kwi-dentin mineralization yaphandwa.Iigundane (i-100 g) zifakwe ngaphantsi kwe-HEBP (8 mg P / kg) kwi-7 okanye i-14 d, kwaye i-incisors yaqhutyelwa kwi-Ca histochemistry kunye / okanye i-electron microscopy.I-incisors ephathwa nge-HEBP ibonise i-ladder-like alternate row of mineralized and non-mineralized dentin at the end apical.I-GBHA ibonakalise ukusabela okuphakathi kwe-Ca kwimatrix yedentin engeyiyo iminerali apho i-electron microscopy ibonise usasazo olutyebileyo lwemathiriyeli eshinyeneyo efana ne-electron-mesh.Imatrix ye-dentin ye-mantle engaxutywanga neminerali yayingalunganga kwi-Ca kodwa iqulethe ii-matrix vesicles (MVs) ezininzi ezizaliswe yikristale kunye/okanye iidipozithi zeminerali e-amorphous.Ukwenziwa kweminerali ye-dentin ye-circumpulpal kwenzeka ngokuzimeleyo ku maleko e-dentin ye-mantle etyebileyo ye-MV kwiisampulu ezichaphazelekayo.Idatha yethu ibonelela ngobungqina be-histochemical bepropathi enokwenzeka ye-Ca-binding ye-circumpulpal dentin matrix kunye nokungabikho kwayo kwi-dentin ye-mantle apho i-MV-mediated mineralization yenzeke khona.Kwi-dentin ye-mantle, i-HEBP ayiphazamisi ukukhula kwekristale kwi-MVs kodwa inqanda ukuphuma kwayo emva kokuphuka kwe-membrane.Kucetywa ukuba i-circumpulpal dentin matrix ibe namandla okwenza iminerali ngokuzimeleyo kwiminerali ye-MV ye-mantle dentin, nangona i-MVs imisela indawo yokuqala kunye nexesha lokuchithwa kwe-dentin mineralization.