Iimveliso

I-Adenosine yi-endogenous regulator ye-vascular tone.Lo msebenzi we-adenosine upheliswa ngokuthathwa kwayo kunye ne-metabolism ngamaseli endothelial microvascular (MVEC).Umthuthi ophambili obandakanyekayo yi-ENT1 (uhlobo lwe-1 olulinganayo lwe-nucleoside transporter).I-MVEC iphinda ivakalise i-nucleobase transporter (ENBT1) echaphazelekayo kwi-cell flux ye-adenosine metabolites efana ne-hypoxanthine.Utshintsho kuyo nayiphi na yezi nkqubo zothutho luya kuba nefuthe kwi-bioactivity ye-adenosine kunye ne-metabolism yayo, kubandakanywa ukubunjwa kwe-oxygen free radicals.I-MVEC eyodwa kwi-skeletal muscle ye-ENT1 (+/+) kunye ne-ENT1 (-/-) iigundane ziphantsi koxinzelelo lwe-oxidative olubangelwa yi-ischemia / i-reperfusion okanye i-menadione.Imisebenzi esebenzayo ye-ENT1 kunye ne-ENBT1 iye yavavanywa ngokusekwe kwi-zero-trans influx kinetics ye-radiolabeleled substrates.Kwakukho ukuhla kwezinga le-ENBT1-mediated hypoxanthine uptake yi-ENT1 (+/+) i-MVEC ephathwa nge-menadione okanye emva kokuvezwa kweemeko ezifanisa isc hemia / reperfusion.Kuzo zombini ezi meko, i-superoxide dismutase mimetic MnTMPyP yanciphisa ukulahleka komsebenzi we-ENBT1, ibandakanya i-superoxide radicals kwimpendulo.Ngokwahlukileyo koko, i-MVEC yodwa kwi-ENT1 (-/-) yeempuku ayizange ibonise ukuncitshiswa komsebenzi we-ENBT1 kunyango lwe-menadione okanye i-ischemia efanisiweyo / i-reperfusion, kodwa baye banezinga eliphezulu kakhulu lomsebenzi we-catalase ngokumalunga ne-ENT1 (+/+) i-MVEC.Ezi nkcukacha zibonisa ukuba umsebenzi we-ENBT1 uyancipha kwi-MVEC ekuphenduleni ukunyuka kwe-superoxide radical ehambelana nokulimala kwe-ischemia / ukubuyisela kwakhona.I-MVEC ihlukaniswe kwi-ENT1 (-/-) iigundane azibonisi oku kuncipha kwi-ENBT1, mhlawumbi ngenxa yokwanda komsebenzi we-catalase.