Iodonitrotetrazolium chloride Cas: 146-68-9 98% Umthubi okhanyayo ukuya kumgubo o-orenji
| Inombolo Yekhathalogu | XD90217 |
| Igama lemveliso | Iodonitrotetrazolium chloride |
| CAS | 146-68-9 |
| Ifomula yeemolekyuli | I-C19H13ClIN5O2 |
| Ubunzima beMolekyuli | 505.70 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 32129000 |
Ukucaciswa kweMveliso
| Ukuchongwa | H-NMR |
| Ukunyibilika | Isisombululo esicacileyo |
| Imbonakalo | Umthubi okhanyayo ukuya kumgubo o-orenji |
| Uvavanyo lwe-HPLC | >98% |
Ukuhanjiswa okuphindaphindiweyo kwejini ye-ABL1 kumfuzo we-BCR kubangela ukubonakaliswa kwe-oncogenic BCR-ABL1 fusion protein, ebonakalisa i-myeloid leukemia yabantu (CML), isifo se-myeloproliferative esithathwa njengesibulalayo ngokungaguqukiyo de kwangeniswa usapho lwe-imatinib lwe-tyrosine kinase. ii-inhibitors (TKI).Nangona kunjalo, ukungavani kweeseli ze-CML kunyango lwe-TKI kunye nokuxhathisa okungaphakathi okanye okufunyenweyo zisezizizathu eziqhelekileyo zokuzingisa kwesifo kunye nokuqhubela phambili kwesigaba se-blastic esifunyenwe kwizigulane emva konyango lokuqala oluyimpumelelo.Apha, siye saphanda indima enokwenzeka ye-MAPK15/ERK8 kinase kwi-BCR-ABL1-dependent autophagy, inkqubo ephambili ye-oncogene-induced leukemogenesis.Kulo mongo, sibonise amandla e-MAPK15 ekufuneni ngokwasemzimbeni i-oncogene kwii-autophagic vesicles, eqinisekisa i-hypothesis yethu yendima ebalulekileyo yebhayoloji kule MAP kinase ekuhanjisweni komqondiso yile oncogene.Ewe, ngokwenza imodeli yomqondiso we-BCR-ABL1 kwiiseli ze-HeLa kunye nokufumana inzuzo yemodeli efanelekileyo ye-physiologically ye-CML yomntu, okt iiseli ze-K562, sibonise ukuba i-BCR-ABL1-induced autophagy ilawulwa yi-MAPK15 ngokukwazi kwayo ukusebenzisana ne-LC3-usapho. iiprotheni, ngendlela exhomekeke kwi-LIR.Okuthakazelisayo kukuba, siye sakwazi ukuphazamisa i-BCR-ABL1-induced autophagy ngendlela ye-pharmacological ejoliswe ekuvimbeleni i-MAPK15, ukuvula ithuba lokusebenza kule kinase ukuchaphazela i-autophagy kunye nezifo ngokuxhomekeke kulo msebenzi weselula.Inene, ukuxhasa ukuba nokwenzeka kwale ndlela, sibonise ukuba ukuncipha kwentetho ye-MAPK15 engapheliyo ithintele ukwanda kweseli exhomekeke kwi-BCR-ABL1, kwi-vitro, kunye nokwakheka kwethumba, kwi-vivo, ke ngoko ibonelela ngenoveli "enotywala" ikhonkco phakathi kwe-BCR-ABL1 kunye CML yabantu.
