L-Leucine Cas: 61-90-5 99% Umgubo omhlophe
| Inombolo Yekhathalogu | XD90304 |
| Igama lemveliso | L-Leucine |
| CAS | 61-90-5 |
| Ifomula yeemolekyuli | C6H13NO2 |
| Ubunzima beMolekyuli | 131.17292 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29224985 |
Ukucaciswa kweMveliso
Ngokufana nezinye iintsholongwane ze-RNA ezi-positive-strand, i-tombusviruses iphinda iphindwe yi-membrane-bound bound viral replicase complex (VRC).I-VRC iqulethe i-p92 virus-coded RNA-dependent RNA polymerase (RdRp), i-viral p33 RNA chaperone, kunye neeprotheni ezininzi ezidibeneyo ezikhethiweyo.Ukuze ube yi-RdRp esebenzayo emva kokuguqulelwa kwayo, iprotheni ye-p92 yokuphindaphinda kufuneka ifakwe kwi-VRC, ilandelwe kukusebenza kwayo.Siye sabonisa ngaphambili kwi-cell-free yeast extract-based assay ukuba ukusebenza kwe-Tomato bushy stunt virus (TBSV) RdRp kufuna i-soluble host factor(s).Kweli nqaku, sichonga iprotheyini yokutshatyalaliswa kobushushu beselula 70 (Hsp70) njengento edibeneyo yokusingatha efunekayo ukuze kusebenze i-N-terminally truncated recombinant TBSV RdRp.Ukongeza, ukuvinjwa okusekwe kwimolekyuli encinci ye-Hsp70 kuthintela i-RNA synthesis yi-tombusvirus RdRp in vitro.Ngapha koko, sibonisa ukuba i-phospholipids engathathi hlangothi, oko kukuthi, i-phosphatidylethanolamine (PE) kunye ne-phosphatidylcholine (PC), iphucula ukusebenza kwe-RdRp kwi-vitro.Ngokwahlukileyo, i-phosphatidylglycerol (PG) ibonisa impembelelo eyomeleleyo kunye neyongamileyo yokuthintela kwi-in vitro RdRp kusebenze.Sikwabonisa ukuba i-PE kunye ne-PC zivuselela i-RdRp-viral plus-strand RNA [(+)RNA] ukusebenzisana, ngelixa i-PG inqanda ukubophelela kwe-RNA yentsholongwane kwi-RdRp.Ngokusekwe kwiindima ezikhuthazayo ezichasene ne-inhibitory ze-phospholipids ezahlukeneyo kwi-tombusvirus RdRp activation, sicebisa ukuba ukubunjwa kwe-lipid ye-subcellular membranes ekujoliswe kuyo kusenokusetyenziswa zi-tombusviruses ukulawula indibano entsha ye-VRC ngexesha losulelo. (RdRp), enoxanduva lokudibanisa inzala yentsholongwane ye-RNA kwiiseli ezosulelekileyo zeentsholongwane ezininzi ze-RNA ezinomtya, ayisebenzi ekuqaleni.Esi sicwangciso sinokuthi sithintele i-viral RNA synthesis kwi-cytosol eya kukhokelela ngokukhawuleza ekufakweni kweempendulo ze-antiviral zeselula ezibangelwa yi-RNA.Ngexesha lokudityaniswa kwe-membrane-bound replicase complex, i-viral RdRp iyasebenza ngenkqubo engaqondwanga ngokupheleleyo eyenza i-RdRp ikwazi ukudibanisa i-RNA.Ngokusebenzisa i-TBSV RdRp, sibonisa ukuba i-cellular cell Hsp70 chaperone kunye ne-phospholipids engathathi hlangothi iququzelela ukusebenza kwe-RdRp kwi-vitro.Ngokwahlukileyo, i-phosphatidylglycerol (PG) inefuthe elilawulayo lokuthintela kwi-in vitro RdRp kusebenze kunye ne-RdRp-virus ye-RNA intsebenziswano, icebisa ukuba i-membranous microdomain ejikeleze i-RdRp ichaphazela kakhulu isakhono sayo se-RNA synthesis.Ke ngoko, ukusebenza kwentsholongwane ye-RdRp kunokwenzeka ukuba kuxhomekeke kwizinto ezininzi zomkhosi kwiiseli ezosulelekileyo.
I-4-Nitrophenyl-N-acetyl-β- D-glucosaminide i-substrate eluncedo yovavanyo olukhawulezayo lwe-colorimetric ye-N-acetyl-b-glucosaminidase umsebenzi kumchamo womntu.I-Chromogenic β-Glucosaminidase substrate ivelisa isisombululo esityheli xa siqhekeka, siqeshwe ngokukodwa kwigwele kunye nokungunda.
