Iimveliso

Ubuzaza bokumelana neentsholongwane ezidityaniswe nokunqongophala kwee-antimicrobial ezintsha zonyuse umdla kuphuhliso lwe-antimicrobial peptides (AMPs) njengenoveli yonyango.Kolu phononongo, sivavanye imisebenzi ye-antimicrobial yeepeptide ezimbini ezimfutshane zokwenziwa, ezizezi, i-RRIKA kunye ne-RR.Ezi peptide zibonise umsebenzi onamandla we-antimicrobial ngokuchasene ne-Staphylococcus aureus, kunye nemiphumo yazo ye-antimicrobial yandiswa kakhulu ngokongezwa kwee-amino acids ezintathu kwi-C terminus, eyathi ngenxa yoko yandisa i-amphipathicity, i-hydrophobicity, kunye ne-net charge.Ngaphezu koko, i-RRIKA kunye ne-RR zibonise isiphumo esibalulekileyo nesikhawulezayo se-bactericidal ngokuchasene nekliniki kunye ne-Staphylococcus-resistant-resistant I-Staphylococcus isolate, kubandakanywa ne-methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-intermediate S. aureus (VISA), vancomycin-resistant S. aureus (VRSA) , i-linezolid-resistant S. aureus, kunye ne-methicillin-resistant Staphylococcus epidermidis.Ngokwahlukileyo kwii-AMPs ezininzi zendalo, i-RRIKA kunye ne-RR zigcine umsebenzi wendlalifa phambi koxinzelelo lwe-physiological ye-NaCl kunye ne-MgCl2.Bobabini i-RRIKA kunye ne-RR yandisa ukubulawa kwe-lysostaphin ngaphezu kwe-1,000-fold kwaye yaphelisa i-MRSA kunye ne-VRSA yodwa ngaphakathi kwe-20 min.Ngaphaya koko, iipeptides ezibonisiweyo zaziphezulu ekunciphiseni i-biofilms ebambelelayo ye-S. aureus kunye ne-S. epidermidis xa kuthelekiswa neziphumo ngamayeza aqhelekileyo okubulala iintsholongwane.Iziphumo zethu zibonisa ukuba iziphumo ze-staphylocidal ze-peptides zethu zazihamba nge-permeabilization ye-membrane ye-bacteria, ekhokelela ekuvuzeni komxholo we-cytoplasmic kunye nokufa kweeseli.Ngapha koko, i-peptides yayingeyona inetyhefu kwiiseli ze-HeLa kwi-4- ukuya kwi-8-folding their concentrations of antimicrobial.Imisebenzi ye-antimicrobial enamandla kunye netyuwa ye-peptides ibonisa umgqatswa onomtsalane wonyango wonyango lwe-S. aureus enganyangekiyo ngamachiza amaninzi.