I-MES HEMISODIUM SALT Cas: 117961-21-4 99% Umgubo omhlophe wekristale
Inombolo Yekhathalogu | XD90051 |
Igama lemveliso | IMES I-HEMISODIUM UTFU |
CAS | 117961-21-4 |
Ifomula yeemolekyuli | (C6H12NO4S)2Na |
Ubunzima beMolekyuli | 205.70 |
Iinkcukacha zokuGcina | I-Ambient |
Ukucaciswa kweMveliso
Imbonakalo | Umgubo omhlophe wekristale |
Ugcino Temp | Gcina eRT |
Isivavanyi | 99% |
I-MES Buffer ichaphazela i-Arabidopsis Root Apex Zonation kunye nokuKhula kweeNgcambu ngokucinezela isiZukulwana se-Superoxide kwi-Root Apex
Kwizityalo, ukukhula kweengcambu kunye neenwele zeengcambu kulawulwa ngulawulo oluhle lweselula ye-pH kunye neentlobo ze-oxygen esebenzayo (ROS).I-MES, i-2-(N-morpholino) i-ethanesulfonic acid njengenye yezithinteli ze-Good's isetyenziswe ngokubanzi kwisithinteli se-buffering medium, kwaye kucingelwa ukuba ilungele ukukhula kwezityalo kunye noxinzelelo kwi-0.1% (w/v) ngenxa yokuba umthamo we-buffer I-MES ukusuka kwi-pH 5.5-7.0 (ye-Arabidopsis, i-pH 5.8).Nangona kunjalo, iingxelo ezininzi zibonise ukuba, ngokwendalo, iingcambu zifuna amaxabiso ahlukeneyo e-pH kumphezulu wezowuni ezithile zeengcambu, ezizezi, i-meristem, indawo yotshintsho, kunye nendawo yobude.Ngaphandle kwenyani yokuba iingcambu zihlala zikhula kumajelo eendaba aqulathe imolekyuli yesithinteli, kuncinci okwaziwayo ngempembelelo ye-MES ekukhuleni kweengcambu.Apha, siye sajonga iziphumo zokugxila okwahlukeneyo kwe-MES buffer kusetyenziswa iingcambu ezikhulayo ze-Arabidopsis thaliana.Iziphumo zethu zibonisa ukuba i-1% ye-MES ithintele kakhulu ukukhula kweengcambu, inani leenwele zeengcambu kunye nobude be-meristem, kanti i-0.1% ikhuthaze ukukhula kweengcambu kunye nommandla wengcambu (ingingqi eqala ukusuka kwincam yengcambu ukuya kwindawo yenguqu).Ngaphaya koko, imveliso ye-superoxide kwi-root apex yanyamalala kwi-1% ye-MES.Ezi ziphumo zibonisa ukuba i-MES iphazamisa i-root morphogenesis eqhelekileyo ngokutshintsha i-ROS homeostasis kwi-root apex.