Iimveliso

I-activators ye-Carbonic anhydrase: isifundo sokuqala sokuvula i-isoform VI yemfihlo yomntu kunye ne-amino acids kunye ne-amines.

I-secretory isozyme ye-carbonic anhydrase yabantu (hCA, EC 4.2.1.1), i-hCA VI, iye yaqulunqwa, ibonakaliswe, kwaye yahlanjululwa kwindlela yokubonakalisa ibhaktheriya.I-kinetic parameters ye-CO(2) hydration reaction ibonise ukuba i-hCA VI ine-ak(ikati) ye-3.4 x 10(5)s(-1) kunye ne-k(ikati)/K(M) ye-4.9 x 10(7)M. (-1) s(-1) (kwi-pH 7.5 kunye ne-20 degrees C).I-hCA VI inomsebenzi obalulekileyo we-catalytic we-physiological reaction, yomyalelo ofanayo wobukhulu njenge-isoform ye-CA I okanye i-transmembrane, i-tumor ehambelana ne-isozyme CA IX.Uluhlu lwama-amino acids kunye nee-amines luboniswe ukuba lusebenze njenge-activators ze-CA VI, kunye nokusebenza okuguquguqukayo.I-l-His, i-l-Trp, kunye ne-dopamine ibonise i-CA VI ebuthathaka i-activating effects (K (A) s kuluhlu lwe-21-42 microM), kanti i-d-His, d-Phe, l-DOPA, l-Trp, i-serotonin , kunye nezinye i-pyridyl-alkylamines bezisebenza ngcono, kunye ne-K (A) kuluhlu lwe-13-19 microM.I-CA VI i-activators engcono kakhulu yayiyi-l-Phe, d-DOPA, l-Tyr, 4-amino-l-Phe, kunye ne-histamine, kunye ne-K (A) kuluhlu lwe-1.23-9.31 microM.Zonke ezi zivuseleli ziphucula i-k(ikati), ezingenasiphumo kwi-K(M), zithatha inxaxheba ngoko kumyinge wokumisela inyathelo kumjikelo we-catalytic, impendulo yokudluliselwa kweproton phakathi kwesiza esisebenzayo se-enzyme kunye nokusingqongileyo.