Iimveliso

Isetyenziselwa ukwenza isithinteli seCAPS, isithinteli sezwitterionic esiluncedo kuluhlu lwe-pH 7.9-11.1.I-CAPS buffer isetyenziswa kakhulu kwi-Western and immunoblotting experiments kunye nolandelelwano lweprotheyini kunye nokuchongwa.Isetyenziswe kwi-electro transfer of proteins to PVDF (sc-3723) okanye i-nitrocellulose membranes (sc-3718, sc-3724).I-pH ephezulu yesi sithinteli yenza kube luncedo ekugqithiseni iiprotheni nge-pI> 8.5.kunye ne-reactivity encinci kunye ne-enzymes okanye iiprotheni, iziphumo ezincinci zetyuwa.

Kwi-capillary zone electrophoresis i-electrophoretic velocity ye-ion iyancipha njengoko ukuxinwa kwesisombululo se-electrolyte yangasemva kunyuswa.Oku kubangelwa utshintsho kwi-electrophoretic mobility of ion (muep) kunye notshintsho kumandla ombane achaphazelayo, oko kukuthi amandla ombane asebenzayo (Eeff).Ukuhamba kwe-electrophoretic ye-ion kuguqulwa ngotshintsho kwi-viscosity epheleleyo yesisombululo se-electrolyte kunye notshintsho kubungakanani obunyibilikisiweyo be-ion.I-Eeff iguqulwa ikakhulu ngotshintsho kubungakanani bentlawulo ye-asymmetry effect kunye nefuthe le-electrophoretic, zombini ezibuyisela ukunyakaza kwee-ion.Kulo cwaningo, ubuchule bokumakisha abathathu busetyenziselwa ukufundisisa umphumo wokugxininiswa kwe-electrolyte yangasemva (0.02-0.08M 3-[cyclohexylamino] -1-propanesulfonic acid kunye ne-counter ion (Li, Na, K, kunye ne-Cs) kwi-Eeff. Kwafunyaniswa ukuba ugxininiso lwe-electrolyte yangasemva luyichaphazela ngokuphawulekayo i-Eeff kwaye i-Eeff isondela ku-E njengoko uzinzo lwe-electrolyte yangasemva lusondela ku-zero I-ion yekhawunta ibenempembelelo encinci kwi-Eeff: njengoko ubukhulu berediyasi ye-hydrated ye-counter ion yanda. , i-Eeff yehlile.Ubuchule bokuphawula ezintathu bungqineke busebenza kakuhle kumiselo olunjalo.