i-oomassie eluhlaza okwesibhakabhaka eqaqambileyo R-250 Cas: 6104-59-2 Blue ukuya kumgubo omnyama oluhlaza
| Inombolo Yekhathalogu | XD90541 |
| Igama lemveliso | oomassie bhlowu eqaqambileyo R-250 |
| CAS | 6104-59-2 |
| Ifomula yeemolekyuli | I-C45H44N3NaO7S2 |
| Ubunzima beMolekyuli | 825.97 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 32129000 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo oluhlaza okwesibhakabhaka ukuya kumnyama omnyama |
Sifunde ukuchithwa kwemibala ye-malachite eluhlaza (MG) yi-fungus Cunninghamella elegans.Umsebenzi we-mitochondrial wokunciphisa iMG wanda ngokunyuka kwangaxeshanye kwiprotheni ye-9-kDa, ebizwa ngokuba yiCeCyt.Ubukho be-cytochrome c kwiprotheni ye-CeCyt yamiselwa yi-optical absorbance spectroscopy ene-extinction coefficient (E(550-535)) ye-19.7+/-6.3 mM(-1) cm(-1) kunye namandla okunciphisa + 261 mV.Xa i-CeCyt ecocekileyo yongezwa kwi-mitochondria, umsebenzi othile we-CeCyt ufikelele kwi-440 +/- 122 micromol min (-1) mg (-1) iprotheni.Ukuthintelwa kokunciphisa iMG nge-stigmatellin, kodwa kungekhona nge-antimycin A, kubonise unxibelelwano olunokwenzeka lomsebenzi we-CeCyt kwindawo ye-Qo ye-bc1 complex.Iziphumo ze-RT-PCR zibonise ukulawulwa okuqinileyo kwe-cecyt gene expression nge-oxygen esebenzayo.Sicebisa ukuba i-CeCyt isebenze njengeprotheyini yokunciphisa i-MG phantsi koxinzelelo lwe-oxidative kwinqanaba elimileyo okanye lesibini lokukhula kwale fungus.2009 Elsevier Inc. Onke amalungelo agciniwe.
