p-Nitrophenyl -aL-Fucopyranoside Cas: 10231-84-2 Imhlophe ukuya kumthubi ikristale powder
| Inombolo Yekhathalogu | XD90142 |
| Igama lemveliso | p-Nitrophenyl -aL-Fucopyranoside |
| CAS | 10231-84-2 |
| Ifomula yeemolekyuli | C12H15NO7 |
| Ubunzima beMolekyuli | 285.25 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 29400000 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo wekristale omhlophe ukuya kumthubi |
| uAsay | 99% |
| TLC | Indawo enye |
| Ubunyulu HPLC | Ubuncinci 98% |
| Ukunyibilika (1 % emanzini) | Sula isisombululo esingenambala |
| Ukuxinana | 1.503±0.06 g/cm3 (20 ºC 760 Torr), |
| Indawo yokunyibilika | 196-197 ºC |
| Indawo yokubilisa | 515.4°Cat760mmHg |
| Indawo yokuflasha | 265.5°C |
| Isalathiso sokuqhafaza | 1.623 |
I-LecA (PA-IL) yi-lectin ye-cytotoxic kunye ne-adhesin eveliswa yi-Pseudomonas aeruginosa ebophelela i-hydrophobic galactosides kunye nokuchaneka okuphezulu kunye nobudlelwane.Ngokusebenzisa i-lecA-egfp translation fusion kunye nohlalutyo lwe-immunoblot ye-biofilm extracellular matrix, sibonisa ukuba i-lecA ibonakaliswe kwiiseli ezikhulile ze-biofilm.Kwiimvavanyo ze-static biofilm kuzo zombini i-polystyrene kunye nentsimbi engatyiwa, ubunzulu be-biofilm kunye nokugutyungelwa komphezulu kuye kwacuthwa ngokuguqulwa kwe-lecA kwaye kwandiswa kuhlobo lwe-LecA-okuvelisa ngokugqithisileyo i-PAO-P47.I-Biofilm i-surface coverage yi-strain yabazali, i-PAO-P47 kodwa kungekhona i-lecA eguqukileyo kwiikhuphoni zensimbi nayo yathintelwa kukukhula kubukho be-isopropyl-beta-D-thiogalactoside (IPTG) okanye i-p-nitrophenyl-alpha-D-galactoside ( NPG).Ngapha koko, ii-biofilms zasendle ezivuthiweyo ezenziwe ngokungabikho kwezi galactosides ze-hydrophobic zinokusasazwa ngokongezwa kwe-IPTG.Ngokwahlukileyo, ukongezwa kwe-p-nitrophenyl-alpha-L-fucose (NPF) enobudlelwane obuphezulu be-P. aeruginosa LecB (PA-IIL) i-lecti n ayinayo impembelelo ekubunjweni kwe-biofilm okanye ukusabalalisa.Ukukhula kwePlanktonic ye-P. aeruginosa PAO1 ayizange ichatshazelwe bubukho be-IPTG, NPG okanye i-NPF, kwaye i-strain ayizange ikwazi ukusebenzisa ezi swekile njengemithombo yekhabhoni, ebonisa ukuba iziphumo eziphawulweyo ekwenziweni kwe-biofilm kwakungenxa yokuthintela ukhuphiswano lwe-LecA-ligand. ukubopha.Iziphumo ezifanayo nazo zafunyanwa kwii-biofilms ezikhuliswe phantsi kweemeko zokuhamba okuguquguqukayo kwiikhuphoni zentsimbi, ezibonisa ukuba iLecA inegalelo kwi-P. aeruginosa biofilm architecture phantsi kweemeko ezahlukeneyo zokusingqongileyo.
I-alpha-L-fucosidase (EC 3.2.1.51) ikwazi ukukhulula i-t-fucosyl i-residu kwi-chain chain ye-xyloglucan oligosaccharides ifunyenwe kumagqabi ezityalo ze-Arabidopsis.Ngaphezu koko, i-alpha-L-fucosidase ene-substrate ecacileyo efanayo yacocwa kwikhaphetshu (Brassica oleracea) amagqabi ukuze inike iqela elinye kwi-SDS-PAGE.Ulandelelwano lweepeptide ezimbini zifunyenwe kule bhendi yeprotheni, kwaye zazisetyenziselwa ukuchonga i-Arabidopsis gene coding ye-alpha-fucosidase esiyicebisa ukubiza i-AtFXG1.Ukongeza, i-Arabidopsis gene ene-homology ene-alpha-L-fucosidases eyaziwayo nayo ifunyenwe, kwaye sicebise ukuyibiza ngokuba yi-AtFUC1.Zombini i-AtFXG1 kunye ne-ATFUC1 zavezwa ngokugqithisileyo kwiiseli ze-Pichia pastoris kunye nemisebenzi ye-alpha-L-fucosidase efihliweyo kwinkcubeko yenkcubeko.I-alpha-L-fucosidase ekhowudiweyo yi-AtFXG1 yayisebenza ngokuchasene ne-oligosaccharides esuka kwi-xyloglucan XXFG kunye ne-2'-fucosyl-lactitol kodwa kungekhona ngokuchasene ne-p-nitrophenyl-alpha-L-fucopyranoside.Nangona kunjalo, i-AtFUC1 ebonakaliswe ngokungafaniyo yayisebenza kuphela ngokuchasene ne-2'-fucosyl-lactitol.Ke, eyokuqala kufuneka ihambelane ne-xyloglucan metabolism.
