IMIbhobho Cas: 5625-37-6 White crystalline powder 99% ABTS DIAMMONIUM UTWETHWE ULTRA UBANGANGO
| Inombolo Yekhathalogu | XD90117 |
| Igama lemveliso | IMIbhobho (iPiperazine-1,4-bis(2-ethanesulfonic acid) |
| CAS | 5625-37-6 |
| Ifomula yeemolekyuli | I-C8H18N2O6S2 |
| Ubunzima beMolekyuli | 302.37 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 2933599 |
Ukucaciswa kweMveliso
| Iintsimbi ezinzima | <5ppm |
| Ilahleko ekomisweni | <1.0% |
| Ukunyibilika | Isisombululo esicacileyo, esingenambala (5% 1N NaOH) |
| Isivavanyi | 99 - 101% |
| Imbonakalo | Umgubo omhlophe wekristale |
IMIBHAYIBHI [ipiperazine-N,N′-bis(2-ethanesulfonic acid)] isoloko isetyenziswa njengesixhobo esithintelayo kwi-biochemistry.Yi-ethanesulfonic acid buffer ephuhliswe ngu-Good et al.ngeminyaka yee-1960.I-PIPES ine-pKa kufutshane ne-pH yefiziyoloji eyenza ukuba ibe luncedo kumsebenzi wenkcubeko yeeseli.Kubhalwe phantsi ukuze kuncitshiswe ilahleko ye-lipid xa i-buffering glutaraldehyde histology kwizityalo nezilwanyana.
I-peptide ye-antimicrobial i-gainin i-2 yenza ii-pores kwi-lipid membranes kwaye yenza ukuba i-membrane ingene kwimixholo yeselula.Nangona oku kugqobhoza kusengoyena nobangela womsebenzi wayo we-bactericidal, indlela yokwenziwa kwepore ihlala ingaqondwa kakuhle.Ngoko ke siphande ngokweenkcukacha ukusebenzisana kwe-mainin 2 kunye ne-lipid membranes usebenzisa i-unilamellar vesicles enkulu (i-GUVs).Ukubophelela kwe-mainin 2 kwi-lipid membrane ye-GUVs kwandisa utshintsho lwe-fractional kwindawo ye-membrane, i-δ, eyayilingana ne-concentration ye-surface ye-maginin 2, X. Oku kubonisa ukuba izinga eliqhubekayo le-mainin 2-induced two -inguqu yelizwe ukusuka kwimeko engaguqukiyo ukuya kwipore state inyuke kakhulu ngokunyuka kwe δ.Uxinzelelo lwe-lipid membrane elandela ulangazelelo lwe-GUV luphinde lwasebenza i-mainin 2-induced pore formation.Ukutyhila indawo ye-mainin 2, ukusebenzisana kwe-carboxyfluorescein (CF) -elebhile i-gainin 2 (CF-magainin 2) kunye ne-GUVs enye equlethe i-fluorescent probe e-soluble water-soluble, i-AF647, iphandwa ngokusebenzisa i-microscopy ye-confocal.Ukungabikho koxinzelelo ngenxa yolangazelelo, emva kokudibana kwe-main 2 intensity ye-fluorescence ye-GUV rim ngenxa ye-CF-magainin 2 yanda ngokukhawuleza ukuya kwixabiso elizinzileyo, elahlala lingatshintshi ixesha elide, kwaye kwi-4-32 s ngaphambili. Ukuqala kokuvuza kwe-AF647 ukuqina kwerim yaqala ukunyuka ngokukhawuleza ukuya kwelinye ixabiso elizinzileyo.Ngokwahlukileyo koko, phambi koxinzelelo, akukho kunyuka kokuqina kwerim ngaphambi nje kokuba kuqalwe ukuvuza.Ezi ziphumo zibonisa ukuba i-main 2 ayikwazi ukufuduka ukusuka ngaphandle ukuya kwi-monolayer yangaphakathi kude kube ngaphambi kokubunjwa kwepore.Ngokusekelwe kwezi ziphumo, sigqiba ukuba i-mainin 2-induced pore yi-stress-activated pore kunye nokwelula kwe-monolayer yangaphakathi yeyona nto iphambili yokuqhuba ukubunjwa kwepore.
