Iprotein Kinase Supplement KT5823 CAS:126643-37-6
| Inombolo Yekhathalogu | XD90398 |
| Igama lemveliso | Protein Kinase Supplement KT5823 |
| CAS | 126643-37-6 |
| Ifomula yeemolekyuli | I-C29H25N3O5 |
| Ubunzima beMolekyuli | 495.53 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 29349990 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo omhlophe |
I-ATP-gated ye-P2X3 i-receptors ibalulekile i-transducers ye-nociceptive stimuli kwaye iphantse ibonakaliswe ngokukodwa yi-sensory ganglion neurons.Kwi-mouse trigeminal ganglion (TG), umsebenzi we-P2X3 we-receptor uphuculwe ngokungalindelekanga ngebhloko ye-pharmacological ye-natriuretic peptide receptor-A (NPR-A), echaza indima enokuthi inhibitory ye-endogenous natriuretic peptides kwi-nociception edibeneyo yi-P2X3 receptors.Ukungabikho kotshintsho kwi-protein ye-P2X3 ibonisa ukumodareyitha okuntsokothileyo apho iindlela zokunciphisa umsebenzi we-P2X3 we-receptor zihlala zingacacanga. Ukucacisa le nkqubo kwiinkcubeko ze-TG zemouse, sicinezele umqondiso we-NPR-A kunye nokuba yi-siRNA ye-agonist endogenous BNP, okanye i-NPR-A. blocker anantin.Ke ngoko, siphande utshintsho kwi-P2X3 yokusasazwa kwe-receptor kwi-lipid raft membrane compartment, imeko yabo ye-phosphorylation, kunye nomsebenzi wabo wokubamba i-patch clamping.Ukuqala okulibaziseka kwe-P2X3 ye-desensitization yayiyenye indlela yokuphucula okubangelwa yi-anantin yomsebenzi we-P2X3.I-Anant kwisicelo ibangele ukunikezelwa kwakhona kwe-P2X3 ye-receptor kwi-lipid raft compartment kunye nokunciphisa i-P2X3 serine phosphorylation, izinto ezimbini ezingaxhomekekanga.I-inhibitor ye-cGMP exhomekeke kwiprotein kinase kunye ne-siRNA-mediated knockdown ye-BNP ilinganise umphumo we-anantin.Sibonise ukuba kwi-mouse trigeminal neurons endogenous BNP isebenza kwi-NPR-A receptors ukumisela ukudakumba okubalulekileyo kwe-P2X3 receptor function.I-Tonic inhibition ye-P2X3 ye-receptor umsebenzi nge-BNP / NPR-A / PKG iindlela zenzeke ngeendlela ezimbini ezihlukeneyo: i-P2X3 i-serine phosphorylation kunye nokwabiwa kwakhona kwe-receptor kumacandelo angenayo i-raft membrane.Le ndlela inoveli yolawulo lwe-receptor ingaba yithagethi kwizifundo zexesha elizayo ezijolise ekunciphiseni umsebenzi we-P2X3 we-receptors we-dysregulated kwintlungu engapheliyo.
