Proteinase K Cas: 39450-01-6 Umgubo omhlophe
| Inombolo Yekhathalogu | XD90191 |
| Igama lemveliso | Iprotheni K |
| CAS | 39450-01-6 |
| Ifomula yeemolekyuli | I-C29H27N2O12P |
| Ubunzima beMolekyuli | 626.50 |
| Iinkcukacha zokuGcina | -15 ukuya -20 °C |
| IKhowudi yoMrhumo eHarmonized | 35079090 |
Ukucaciswa kweMveliso
| Inombolo Yekhathalogu | XD90191 |
| Igama lemveliso | Iprotheni K |
| CAS | 39450-01-6 |
| Ifomula yeemolekyuli | I-C29H27N2O12P |
| Ubunzima beMolekyuli | 626.50 |
| Iinkcukacha zokuGcina | -15 ukuya -20 °C |
| IKhowudi yoMrhumo eHarmonized | 35079090 |
Kwizilwanyana ezininzi ezineechromosome zesini ze-X kunye no-Y, iindlela ezibanzi zechromosome zisetyenziselwa ukulinganisa i-X-linked gene expression kumadoda nabasetyhini.Kwimpukane i-Drosophila melanogaster, indlela yembuyekezo yedosi ikwanwenwela ngokubanzi kwi-X-linked transgenes.Ngaphezulu kwemigca engama-70 eguquguqukayo yegusha yase-Australia i-blowfly i-Lucilia cuprina yenziwe njengenxalenye yomzamo wokuphuhlisa iintlobo zamadoda kuphela kwinkqubo yolawulo lwemfuzo yesi sitshabalalisi siphambili seegusha.Yonke imigca iphethe ijini ephawulweyo yeprotheyini yefluorescent.Kuyo yonke imigca ye-X eqhagamshelwe kwi-X, imibungu yabasetyhini ibonisa ukukhanya okukhanyayo kunemibungu yamadoda, icebisa ukuba ijini ye-marker ayihlawulwanga.Oku kuye kwaqinisekiswa ngobungakanani be-RT-PCR kwimigca ekhethiweyo.Ukufumanisa ukuba i-endogenous X-linked genes idosi ibuyekeziwe, siye sakhetha i-8 yemfuza eyi-orthologs yemfuza ekwi-chromosome yesine kwi-D. melanogaster.Ubungqina bamva nje bubonisa ukuba i-D. melanogaster ichromosome yesine, okanye i-Muller element F, yi-chromosome ye-X yekhokho kwi-Diptera ebuyele kwi-autosome kwiintlobo ze-Drosophila.Sibonisa nge-PCR yobuninzi be-DNA yamadoda nabasetyhini ukuba i-6 ye-8 yeqela lonxibelelano ye-F yemfuza ihlala kwi-X chromosome kwi-L. cuprina.Ezinye ezimbini ze-Muller element F zifunyenwe ziyi-autosomal kwi-L. cuprina, kanti ezimbini ze-Muller element B zifunyenwe kwingingqi enye ye-X chromosome njenge-L. cuprina orthologs ye-D. melanogaster Ephrin kunye ne-gawky genes.Sifumanisa ukuba i-L. cuprina X yejini yekhromozomu ibonakaliswa ngokulinganayo kumadoda nabasetyhini (oko kukuthi, idosi ihlawulelwe ngokupheleleyo).Ngaloo ndlela, ngokungafaniyo ne-Drosophila, kubonakala ngathi inkqubo yembuyekezo yedosi ye-Lucilia ikhethekileyo kwiimfuza ezingapheliyo kwi-chromosome ye-X kwaye ayikwazi ukukhethwa yi-transgenes esanda kufika.
