N,N'-Methylenebisacrylamide Cas: 110-26-9 98% Umgubo omhlophe
| Inombolo Yekhathalogu | XD90231 |
| Igama lemveliso | N,N'-Methylenebisacrylamide |
| CAS | 110-26-9 |
| Ifomula yeemolekyuli | I-C7H10N2O2 |
| Ubunzima beMolekyuli | 154.1665 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 29241900 |
Ukucaciswa kweMveliso
| Isivavanyi | >98% |
| Amanzi anganyibilikiyo | <0.5% |
| Imbonakalo | Umgubo omhlophe |
| Iinorganic sulfates | <0.5% |
| Isetyenziselwa uphando kuphela, hayi ukusetyenziswa komntu | uphando lusetyenziselwa kuphela, kungekhona ukusetyenziswa komntu |
Indima ebalulekileyo ye-tumor-associated fibroblasts (TAF) ekuqhubekeni komhlaza ngoku icacile kumhlaza wemiphunga weseli ongeyonxalenye encinci (NSCLC).Nangona kunjalo, unyango oluchasene ne-TAF lulinganiselwe ngenxa yokunqongophala kokuqonda kwiindlela ezikhethekileyo eziphantsi kokuqokelela kwazo.Apha, i-mechanical (okt, i-matrix rigidity) kunye ne-soluble mitogenic cues eqhuba ukuqokelela kwe-TAFs ukusuka kwii-subtypes ezinkulu ze-NSCLC: i-adenocarcinoma (ADC) kunye ne-squamous cell carcinoma (SCC) yachithwa.I-Fibroblasts yakhuliswa kwi-substrata yobunjineli ukubonisa ukuqina okuqhelekileyo-okanye kwe-tumor-efana ne-tumor kwiindawo ezahlukeneyo ze-serum, kwaye iinkqubo zokulawula ezibalulekileyo zacaciswa.Ekulawuleni i-fibroblasts ezivela kwizicubu ezingabonakaliyo, ukuqina kwe-matrix yodwa kwandisa i-fibroblast accumulation, kwaye le mpembelelo yomatshini yayinamandla okanye ithelekiseka kunye neyokukhula kwezinto ezinyibilikayo ukuya kwi-0.5% serum.Iimpawu ezikhuthazayo zokuqina kwe-matrix ziqhutywa yi-β1 integrin mechano-sensing ngokusebenzisa i-FAK (pY397), kwaye yayanyaniswa ne-posttranscriptally eqhutywa ukunyuka kwi-β1 integrin expression.Isiphaluka sokugqibela se-mechano-regulatory siphinde sabonwa kwii-TAF kodwa kwi-subtype-specific fashion, kuba i-SCC-TAF ibonise i-FAK ephezulu (pY397), i-β1 ibonakaliso, kunye ne-ERK1 / 2 (pT202 / Y204) kune-ADC-TAFs.Ngaphezu koko, ukuqina kwe-matrix kubangele uqokelelo olukhulu lwe-TAF kwii-SCC-TAFs (>50%) xa kuthelekiswa nee-ADC-TAFs (10% -20%).Ngokwahlukileyo koko, ii-SCC-TAFs ubukhulu becala ziye zaphelelwa yi-serum, ngelixa ii-ADC-TAFs zasabela kuxinzelelo oluphezulu lweserum kuphela.Ezi ziphumo zibonelela ngobungqina bokuqala bolawulo oluthile lwe-NSCLC-TAF yokuqokelela.Ngapha koko, ezi nkcukacha zixhasa ukuba unyango olujolise ekubuyiseleni ukuqina kwemiphunga kunye/okanye i-β1 integrin-dependent mechano regulation inokusebenza ngokuchasene ne-SCC-TAFs, kanti ukuthintela ukukhula kwestromal factor signaling inokusebenza ngokuchasene ne-ADC-TAFs. Ukuqokelelana okungaqhelekanga kwee-fibroblasts ezixhasa ithumba kwiintlobo ezimbini ezinkulu zomhlaza wemiphunga, eziya kunceda uphuhliso lonyango lobuqu ngokuchasene nezi seli.
