Iimveliso

I-microRNA (miRNA) biosensor enovakalelo kakhulu esebenzisa i-ruthenium oxide nanoparticle (RuO(2) NP)-iqaliswe ukwenziwa kwepolymerization ye-3,3'-dimethoxybenzidine (DB) kunye ne-miRNA-templated deposition ye-insulating poly(3,3'-dimethoxybenzidine) (PDB) ifilimu ichazwe kulo msebenzi.I-biosensor yenziwe nge-monolayer edibeneyo ye-oligonucleotide yokubamba i-probes (CPs) kunye ne-4-mercaptoaniline kwi-electrode yegolide.Ukulandela ukuxutywa nge-RuO (2) ye-NP-tagged target miRNA, umxube we-DB/H(2)O(2) kwi-pH 5.0 0.10 M i-acetate buffer isetyenziswe kwi-biosensor.I-RuO (2) NPs isebenza njenge-polymerization initiator / catalyst ye-polymerization ye-DB.Kwaye imicu ye-anionic miRNA exutyiweyo kunye neeCPs zasimahla zisebenza njengeetemplates, ezikhokela ukubekwa kwePDB.Isixa sePDB ediphozithiweyo kunye namandla ayo okukhusela anxulumana ngokuthe ngqo kugxininiso lwethagethi ye-miRNA kwisisombululo.Iimvavanyo ze-electrochemical impedance spectroscopic zibonise ukuba i-linear charge-transfer resistance-concentration relationship ukusuka kwi-6.0 fM ukuya kwi-2.0 pM ifunyenwe emva kwe-60 min yokufukanyelwa kumxube we-DB/H (2)O (2).Kwakungekho-cross-hybridization phakathi kwe-pre-miRNA kunye ne-miRNA evuthiweyo kunye ne-cross-hybridization encinci kakhulu phakathi kwamalungu osapho lwe-miRNA asondeleleneyo nakwinqanaba elinye lesiseko-esingafaniyo.Le biosensor isekwe kwi-impedance ibonelela ngenye indlela enomtsalane ye-miRNA yokuchaza iprofayili kwaye inokwenza uphuhliso lwenkqubo yeprofayili ye-miRNA ephathekayo ephathwayo.