(S) -2-Amino-4-hydroxybutanoic acid Cas: 672-15-1
| Inombolo Yekhathalogu | XD90286 |
| Igama lemveliso | (S) -2-Amino-4-hydroxybutanoic acid |
| CAS | 672-15-1 |
| Ifomula yeemolekyuli | C4H9NO3 |
| Ubunzima beMolekyuli | 119.11916 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29225000 |
Ukucaciswa kweMveliso
| Isivavanyi | 99% |
| Imbonakalo | Umgubo omhlophe |
Uphononongo lwangoku luchaza indlela elula nesebenzayo esebenzisa ukusebenza okuphezulu kwe-chromatography yolwelo (HPLC) edityaniswe nobhaqo lwe-fluorescence ukumisela iiparamitha zekinetic ye-glutamate uptake kwizihlunu zemithambo-luvo.Izicubu ze-retinal ezifunyenwe kumantshontsho eentsuku ezi-7 zifakwe kwi-concentrations eyaziwayo ye-glutamate (50-2000 μM) kwimizuzu eyi-10, kwaye amanqanaba e-o-phtaldehyde (OPA) -i-neurotransmitter ephuma kwi-incubation medium alinganiswe.Ngokuvavanya umahluko phakathi kokugxilwa kokuqala kunye nokugqibela kwe-glutamate phakathi, indlela yokufumana i-saturable ibonakaliswe (K (m) = 8.2 kunye ne-V (max) = 9.8 nmol / mg protein / min).Lo mlinganiso ubukhulu becala wawuxhomekeke kwisodium kunye nobushushu, uxhasa ngamandla ukuba indlela yokuncipha koxinzelelo ngokwenene ithathwa ngabathuthi abanobudlelwane obuphezulu.Ukongeza koku, iziphumo zethu zikwabonise ukuba i-zinc chloride (i-inhibitor ye-glutamate / aspartate transporters) ikhuphe ukuhla okuxhomekeke kwi-concentration kwi-glutama te uptake, ebonisa ubuchule bendlela yethu.Ngokubanzi, umsebenzi okhoyo ubonisa enye indlela yokuvavanya ukuthambekela kwe-glutamate kwizicubu zemithambo-luvo usebenzisa i-HPLC.Le ndlela ingaba sisixhobo esibalulekileyo kwizifundo ezinxulumene nokubonakaliswa kokuguqulwa kwemizuzu ekuthuthweni kwe-glutamate ehambelana nokulimala kwenkqubo ye-nervous central.
