Iimveliso

I-Tricine, yi-zwitterionic buffer reagent egama layo lithatyathwe kwi-Tris kunye ne-glycine.Ukwakhiwa kwayo kufana ne-Tris, kodwa ugxininiso lwalo oluphezulu lunomsebenzi obuthathaka wokuthintela kune-Tris.Enye ye-buffer reagents ye-Good, eyaphuhliswa ekuqaleni ukuze ibonelele ngenkqubo ye-buffer yokusabela kwekloroplast.Uluhlu olusebenzayo lwe-pH buffer ye-Tricine yi-7.4-8.8, pKa=8.1 (25 °C), kwaye iqhele ukusetyenziswa njengesithinteli esiqhubayo kunye nokumisa iipellets zeeseli kwakhona.I-Tricine ineempawu zentlawulo ephantsi engalunganga kunye namandla aphezulu e-ionic, efanelekileyo kakhulu ukuhlukana kwe-electrophoretic yeeprotheni ezinobunzima obuphantsi be-1 ~ 100 kDa.Kwi-firefly luciferase-based assay ATP, ukuthelekisa i-10 buffers eziqhelekileyo, i-Tricine (25 mM) ibonise umphumo ongcono wokufumanisa.Ukongeza, i-Tricine ikwayi-hydroxyl radical scavenger esebenzayo kwimifuniselo yomonakalo we-radical-induced inwebu.