Iimveliso

Ii-reagents ze-Trinder ezintsha ziphuma kwi-aniline enyibilikayo kakhulu emanzini ezisetyenziswa ngokubanzi kwiimvavanyo zokuxilonga kunye novavanyo lwe-biochemical.Kukho iingenelo ezininzi ngaphezu kweerejenti zechromogenic eziqhelekileyo ekumiselweni kombala womsebenzi wehydrogen peroxide.I-reagents entsha ye-Trinder izinzile ngokwaneleyo ukuba isetyenziswe kuzo zombini isisombululo kunye neenkqubo zovavanyo lombhobho.Xa kukho i-hydrogen peroxide kunye ne-peroxidase, i-reagent ye-Trinder inoveli ibonakaliswe ukuba iphendule nge-4-aminoantipyrine (4-AA) okanye i-3-methylbenzothiazole sulfonehydrazone (MBTH) ngexesha le-oxidative coupling reaction.Yenza i-violet ezinzile kakhulu okanye idayi eluhlaza okwesibhakabhaka.I-molar absorbance yedayi edityaniswe ne-MBTH yayiyi-1.5- 2 amaxesha aphezulu kunokuba idayi edibene ne-4-AA;nangona kunjalo, isisombululo se-4-AA sasizinzile ngaphezu kwesisombululo se-MBTH.I-substrate i-enzymatic oxidized yi-oxidase yayo ukuvelisa i-hydrogen peroxide.Uxinzelelo lwe-hydrogen peroxide luhambelana ne-substrate concentration.Ngoko ke, ubungakanani be-substrate bunokugqitywa ngokuphuhliswa kombala we-oxidative coupling reaction.IGlucose, utywala, i-acyl-CoA kunye ne-cholesterol ingasetyenziselwa ukufumanisa ezo substrates zidibene nenoveli ye-Trinder's reagent kunye ne-4-AA.Kukho ii-reagents ze-Trinder ezintsha ezili-10 ezikhoyo.Phakathi kwe-reagents entsha ye-Trinder, i-TOOS yeyona nto isetyenziswa kakhulu.Nangona kunjalo, kwi-substrate ethile, uvavanyo lweeklasi ezahlukeneyo zenoveli ye-Trinder's reagents iyimfuneko ukuphuhlisa eyona nkqubo yokubona.