![Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane Cas: 6976-37-0 99% Umfanekiso ofakiweyo](http://cdn.globalso.com/xdbiochems/6976-37-0.jpg)
Bis [2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane Cas: 6976-37-0 99%
| Inombolo Yekhathalogu | XD90111 |
| Igama lemveliso | I-BIS-TRIS (Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane) |
| CAS | 6976-37-0 |
| Ifomula yeemolekyuli | C8H20NO5 |
| Ubunzima beMolekyuli | 210.2476 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 2922190090 |
Ukucaciswa kweMveliso
| Iintsimbi ezinzima | <10ppm |
| Intsalela kwi-Ignition | <0.1% |
| Pka | 6.3-6.7 |
| Imbonakalo | Umgubo weCrystalline oMhlophe |
| Ukunyibilika (1M kwi-H20) | Icace kwaye iGqibe |
| pH (1M kwi-H2O) | 9.0-11.0 |
| Ukufuma (LOD) | 1.0% ubukhulu. |
| Uvavanyo (Titration kwisiseko esomileyo) | 99.0% min. |
I-Buffer, esetyenziselwa ukukhangela i-creatine-creatinase;ikwasetyenziselwa ukhuseleko olusebenzayo lwe-hemoglobin ekomisweni komkhenkce;catholyte, esetyenziswa kwi-IEF/2-D gel electrophoresis.
I-Glial cell-derived neurotrophic factor (GDNF) sisithinteli esinamandla sokusetyenziswa kwe-ethanol kunye nokuphinda ubuyele, kwaye iigundane ze-GDNF ze-heterozygous knockout zibonisa uvakalelo lomvuzo kwi-ethanol kwaye zisebenzisa i-ethanol eninzi emva kwexesha lokuziyeka kune-littermates yazo yasendle.Apha, siye savavanya ukuba i-ethanol iguqula ukubonakaliswa kwe-GDNF kwindawo ye-ventral tegmental (VTA; indawo ye-GDNF yesenzo) kunye / okanye i-nucleus accumbens (NAc; umthombo oyintloko we-GDNF), kwaye ukuba kunjalo, misela indima ye-endogenous growth factor. kulawulo lokusetyenziswa kwe-ethanol.Ulawulo olucwangcisiweyo lwe-ethanol lwandise ukubonakaliswa kwe-GDNF kunye namanqanaba eprotheyini kwi-VTA, kodwa kungekhona i-NAc.Ukongezelela, amanqanaba e-GDNF aphakanyisiwe emva kweseshoni yokusela i-ethanol kwiigundane ezidla i-ethanol kwi-intermittent-access access two-bottle choice procedure for 1 week, kodwa hayi kwiiveki ezi-7.Ukunyanzeliswa okulandela iiveki ze-7 ze-ethanol ezigqithisileyo zokunciphisa amanqanaba e-GDNF, ngelixa ixesha elifutshane lokusela i-ethanol lokusela emva kokunyanzeliswa kwe-GDNF elawulwayo.Okubalulekileyo, i-knockdown ye-GDNF ngaphakathi kwe-VTA isebenzisa i-adenovirus echaza i-hairpin emfutshane ye-RNA iququzelele ukunyuka kokusela i-ethanol ngeempuku ze-ethanol-naïve, kodwa kungekhona ngeempuku ezinembali yokusetyenziswa kwe-ethanol ngokugqithiseleyo.Ezi ziphumo zibonisa ukuba ngexesha lamava okuqala okusela i-ethanol, i-GDNF kwi-VTA yanda kwaye ikhusela ekuphuhliseni i-ethanol eninzi.Nangona kunjalo, impendulo yokukhusela i-ethanol iyancipha emva kokuthatha ixesha elide kunye nokuhoxiswa kwe-ethanol, okukhokelela ekuqhubekeni, ukusetyenziswa kwe-ethanol ngokugqithiseleyo.
