Iimveliso

I-Bicine yi-zwitterionic amino acid buffer, esebenzayo kuluhlu lwe-pH 7.6-9.0 (pKa ye-8.26 kwi-25 ° C).Isithinteli esicetyiswayo somsebenzi we-biochemical wobushushu obuphantsi.I-Bicine isetyenziselwa ukulungiswa kwesisombululo esizinzileyo se-substrate yokuzimisela kwe-serum guanase.Ukusetyenziswa kwe-bicine kwindlela ecekethekileyo ye-ion yokutshintshiselana ngechromatography yesisombululo seprotheyini ipapashiwe.I-Bicine isetyenziswe kwi-peptide kunye ne-protein crystallization.Uphononongo lwe-kinetic lwe-quaternary transition-state analogue complex ye-creatine kinase isetyenziswe i-bicine kwi-reaction buffer.Inkqubo ye-multiphasic buffer ye-SDS-PAGE yeeprotheni kunye neepeptides ezibandakanya i-bicine ichazwe.

Iindonga ze-alveolar enye eziphantsi kwezifundo zobude boxinzelelo kwi-saline kunye ne-Bicine (0.2 M) zihamba ngokubola okuqhubekayo kwi-tissue tension (TTD).Sihlolisise umphumo wezisombululo ezahlukeneyo kule TTD kwaye sijonge utshintsho oluhambelanayo kwi-ultrastructure.I-Lung parenchyma yacandwa kwiindonga ze-alveolar enye (30 X 30 X 150 microns) kwi-phosphate-buffered saline (0.15 M).Ukutshintshelwa kwindawo yokuhlambela ubude, i-tissue yacwiliswa kwi-Bicine, i-saline, isisombululo se-Hank esiqinisiweyo, i-0.25% ye-Alcian eluhlaza okwesibhakabhaka kwi-saline, okanye isisombululo se-sodium dodecyl sulfate, ngamaxesha ahlukeneyo.Ijikelezwe ngebhayisekile ngolwandiso olunikiweyo ngamandla aphakamileyo alinganiswe ngokuhamba kwexesha, ezi zicubu zifanayo zalungiswa kwi-buffered glutaraldehyde/tannic acid kwaye zisetyenzwa kwi-electron microscopy.Iindonga ze-alveolar enye ezintywiliselwe kwi-saline okanye i-Bicine ibonisa i-TTD eqhubekayo.Iivacuoles okanye izithuba zavela kwi-interstitium apho kunye nokungahambi kakuhle kweselula kwaqhubela phambili neTTD.Kubonwe ngaphakathi kwe-0.3 h, utshintsho luqhubekile kakuhle kwi-0.6 h.Kwi-sodium dodecyl sulfate (70 mM), nangona kunjalo, kwakungekho TTD kwaye ngokwesakhiwo kwakungekho interstitium, kunye ne-basement membranes kuphela kunye neeprotheni ezine-fibrous eziseleyo.Kwisisombululo se-Hank esiqiniweyo okanye i-0.25% ye-Alcian eluhlaza okwesibhakabhaka i-matrix ye-interstitial, i-cell morphology kunye ne-tissue tension yayigcinwe kakuhle kwi-1 h.Olu phononongo lucebisa ukuba ukuvuza kwe-matrix ye-interstitial kwenzeka kwi-saline okanye kwi-Bicine, kwaye i-matrix engaguqukiyo ibalulekile ekugcinweni koxinzelelo lwezicubu.