Iimveliso

Intshayelelo: I-BSA yenye yeeproteni ezixhaphakileyo kwiilabhoratri ze-biochemical, kwaye ukubaluleka kwayo kunokungahoywa kwiimvavanyo kuba ixhaphake kakhulu kwaye ixhaphake kakhulu.I-albumin ye-bovine serum (BSA), eyaziwa ngokuba yinto yesihlanu, i-globulin kwi-bovine serum equlethe i-583 ye-amino acid iintsalela, kunye ne-molecular weight 66.430kDa kunye ne-isoelectric point ye-4.7.I-BSA inoluhlu olubanzi lwezicelo kwiimvavanyo ze-biochemical, ezifana ne-arhente yokuThintela kwiiblothi zasentshona.

Isicelo: I-Albumin ye-Bovine ye-Bovine (BSA), eyaziwa ngokuba yinto yesihlanu, i-globulin kwi-serum ye-bovine, equlethe i-amino acid eyi-607, kwaye inoluhlu olubanzi lwezicelo kwiimvavanyo ze-biochemical.I-albumin ye-bovine ye-serum isetyenziswa ngokubanzi njenge-stabilizer kwisisombululo sokugcina kunye nesisombululo sokusabela se-enzymes yokuthintela okanye i-enzymes eguquliweyo ukuzinzisa umsebenzi we-enzyme kunye nokuthintela ukubola kwe-enzyme kunye ne-adsorption engeyiyo.

Umsebenzi: I-BSA isetyenziswa ngokubanzi njenge-stabilizer kwisisombululo sokugcina kunye nesisombululo sokuphendula se-enzymes yokuthintela okanye i-enzymes eguquliweyo, kuba ezinye i-enzymes azinzile okanye zinomsebenzi ophantsi kwiindawo eziphantsi.Emva kokongeza i-BSA, inokudlala indima "yokukhusela" okanye "umthwali", kwaye umsebenzi wee-enzymes ezininzi unokuphuculwa kakhulu emva kokongeza i-BSA.Ii-Enzymes ezingafuni ukongezwa kwe-BSA azichatshazelwa ngokubanzi kokongezwa kwe-BSA.Uninzi lwe-DNA ye-substrate, i-BSCA inokwenza ukugaya kugqitywe ngakumbi, kwaye inokufikelela ukusika ngokuphindaphindiweyo.Kwi-37 ° C, xa i-reaction ye-digestion idlula i-1 h, i-BSA inokwenza i-enzyme izinzile, kuba kwi-reaction buffer ngaphandle kwe-BSA, i-enzymes ezininzi zokukhawulela zingaphila kuphela kwi-37 ° C kwi-10 ~ 20min okanye ixesha elifutshane..Ngokwahlukileyo koko, i-BSA inokubopha i-ion zetsimbi kunye nezinye iikhemikhali kwi-buffer okanye kwi-substrate DNA ethintela umsebenzi wothintelo lwe-endonucleases.

Ukusetyenziswa: I-albumin ye-serum ye-bovine esemgangathweni (i-BSA, i-StandardGrade), ekwazi ukuhlangabezana neemfuno zeemvavanyo ezininzi zesiqhelo, ezifana ne-immunoblocking agent, i-tissue cell (izilwanyana ezincinci kunye neeseli zezinambuzane, njl.) iireagents kunye nemigangatho yobungakanani beprotheyini.Inqanaba lokuxilonga i-albumin ye-bovine serum (i-BSA, i-DiagnosticGrade) inokuhlangabezana neemfuno ezininzi zovavanyo lwesiqhelo, njenge-agent ye-immunoblocking, i-protein / enzyme stabilizer, i-diluent, i-carrier kunye ne-protein standard.Ukongeza, inokusetyenziselwa uvavanyo lwe-immunoassays, inkcubeko yeeseli kunye novavanyo lwe-hybridization olufuna ubuntununtunu obuphezulu.