I-D-Luciferin Cas: 2591-17-5 99% I-off-white ukuya kwi-powder emthubi nbsp BEETLE LUCIFERIN
| Inombolo Yekhathalogu | XD90248 |
| Igama lemveliso | D-Luciferin |
| CAS | 2591-17-5 |
| Ifomula yeemolekyuli | I-C11H8N2O3S2 |
| Ubunzima beMolekyuli | 280.323 |
| Iinkcukacha zokuGcina | -15 ukuya -20 °C |
| IKhowudi yoMrhumo eHarmonized | 29342080 |
Ukucaciswa kweMveliso
| Umxholo wamanzi | Max.2.0% |
| Unxunguphalo | Ubukhulu be-2.0 NTU |
| Ukujikeleza okuthe ngqo | -36 ukuya -32 |
| Imbonakalo | Okumhlophe-mhlophe ukuya kumgubo omthubi |
| Ubunyulu HPLC | Ubuncinci 99% |
| I-molar extinction coefficient | umz 17900 L/(mol cm) |
Intshayelelo: I-D-luciferin yi-substrate ye-adenosine triphosphate (ATP) exhomekeke kwi-bioluminescence reaction.Umgaqo we-bioluminescence kukuba i-luciferin i-oxidized yi-luciferase phambi kwe-ATP kunye ne-oxygen.Ifomula yokusabela kweekhemikhali ngolu hlobo lulandelayo: ATP+D-Luciferin+O2→Oxyluciferin+AMP+PPi+O2+Light.
Inkqubo yesenzo: Indlela yokusebenza ye-D-luciferin kukuba phantsi kwesenzo se-ATP kunye ne-luciferase, i-luciferin (i-substrate) inokuthi ifakwe i-oxidized ukuze ikhuphe ukukhanya.Xa i-luciferin ingaphezulu, inani leefotoni eziveliswayo lihambelana ngokufanelekileyo kunye noxinzelelo lwe-luciferase.
Isicelo: I-D-luciferin yi-substrate ye-adenosine triphosphate (ATP) exhomekeke kwi-bioluminescence reactions.I-bioluminescence reaction ye-luciferin / luciferase isoloko isetyenziselwa ukufumanisa i-ATP, i-metabolites enokuguqulwa ibe yi-ATP (efana ne-AMP, i-ADP, i-cAMP), kunye ne-enzymes ezinokuvelisa i-ATP (njenge-creatine kinase, njl.), ngoko ke i-bioluminescence reaction ingasetyenziswa.Isebenza ekubhaqweni koluhlu olubanzi lwezinto eziphilayo.
Umsebenzi we-Biological: I-D-Luciferin (i-Firefly luciferin) i-substrate ethandwayo ye-bioluminescence phambi kwe-ATP yokusetyenziswa kwi-luciferase-based bioluminescence imaging kunye ne-cell-based high-throughput screening.
Izifundo ze-in vitro: I-D-luciferin isabela nge-luciferase, i-ATP kunye ne-oksijini ukuze ikhuphe ukukhanya, efunyenwe yifilimu yezithombe ezibucayi ukuze ibone i-alkaline phosphatase-bound antibodies.
Izifundo ze-vivo: ukusetyenziswa kwe-D-luciferin substrates kunye ne-firefly luciferase igcina i-tumor-host i-immune interactions kwi-immunocompetent mouse model kunye nomhlaza we-ovarian, njengoko iinkqubo ze-bioluminescence zivakalelwa ngakumbi ekukhuleni kwethumba kunemiyalelo yokuzuza ubunzima bomzimba.
