I-PMSF Cas: 329-98-6 98.0% i-crystalline powder emhlophe iPhenylmethanesulfonyl fluoride (PMSF)
| Inombolo Yekhathalogu | XD90250 |
| Igama lemveliso | Phenylmethanesulfonyl fluoride (PMSF) |
| CAS | 329-98-6 |
| Ifomula yeemolekyuli | C7H7FO2S |
| Ubunzima beMolekyuli | 174.1927 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29049900 |
Ukucaciswa kweMveliso
| Isivavanyi | ≥98.0% HPLC |
| Imbonakalo | umgubo omhlophe wekristale |
I-PMSF yi-serine/cysteine protease inhibitor engaguqukiyo esetyenziswa ngokuqhelekileyo ekulungiseleleni i-cell lysates.
Izifundo ze-in vitro: I-PMSF (2 mM) inqanda i-carbachol-stimulated inositol phosphate ukuqokelela kuphela i-15% -19% phambi kweLi +.Ukuvinjelwa kwe-phosphoinositide yokuguqulwa kwe-PMSF kubangelwa yinyathelo elinye okanye ngaphezulu emva kokuchithwa kwe-phosphoinositides [1].I-PMSF inqanda i-acylation ye-inositol intsalela ye-GPI ephakathi kwigazi le-T. brucei.I-PMSF inqanda ukubunjwa kwe-glycolipid C kodwa kungekhona i-fatty acid remodeling in vitro.I-PMSF inqanda ukongezwa kwe-GPI acylation kunye ne-ethanolamine phosphatase kwi-procyclic trypanosomes, kodwa kungekhona kwiiseli ze-Hela [2].
Izifundo ze-vivo: I-PMSF (i-0.1 mL / 10 g b.wt, ip) ivelise i-antinociception, njengoko kuboniswe ngokunyuka kwe-dose kwi-%MPE kuvavanyo lwe-latency yomsila, kodwa ayiphumelelanga ukuvelisa i-dose-responsive motor inhibition ecacileyo.Iigundane ezifumene inaliti ye-intraperitoneal ye-PMSF ibonise iziphumo ze-cannabinoid ezibandakanya i-antinociception, i-hypothermia kunye nokungahambi kakuhle kunye ne-ED50 ixabiso le-86, i-224 kunye ne-206 mg / kg, ngokulandelanayo.I-PMSF (30 mg / kg) i-pretreatment iphakamisa imiphumo ye-anandamide kwiimpendulo ze-tail-flick (i-antinociception), umsebenzi we-locomotor kunye nokuhamba nge-5-fold, i-10-fold kunye ne-8-fold, ngokulandelanayo [3].
Uvavanyo lwezilwanyana: Iigundane ze-ICR eziyindoda ezinobunzima be-18 ukuya kwi-25 g zisetyenzisiwe kuvavanyo.I-PMSF yachithwa kwioli yeesame kwaye ilawulwa nge-intraperitoneally kumthamo we-0.1 mL / 10 g b.wt.Hlala ulawula i-PMSF imizuzu eli-10 phambi kwe-anandamide efakwe emthanjeni okanye inaliti yemoto.Iimpuku zaqhelaniswa kwigumbi lovavanyo ngobusuku ngaphandle kokuphazamiseka kokutya okanye amanzi.Ukulandela i-anandamide ye-intravenous okanye ukulawulwa kwesithuthi, isilwanyana ngasinye sihlolwe ngolu hlobo lulandelayo: imizuzu ye-5 yomsila-flick latency (antinociceptive) iimpendulo kunye ne-5 kwimizuzu ye-15 yomsebenzi ozenzekelayo (motor);okanye imizuzu emi-5 yobushushu be-core (rectal) kunye neringi Immobilization (catalepsy) imizuzu emi-5 ukuya kwe-10.
