Iimveliso

I-brucellae zi-etiological agents ze-brucellosis, i-zoonosis esasazwa kwihlabathi jikelele.Ezi bhaktheriya ziyi-facultative intracellular parasites kwaye ngoko ke ziyakwazi ukulungelelanisa imetabolism yazo kwiindawo ezingaphezulu kunye ne-intracellular ezidibana nazo ngexesha lomjikelo wentsholongwane.Nangona kunjalo, le nkalo ye-biology yeBrucella ayiqondwanga ngokugqibeleleyo, kwaye izondlo ezifumaneka kwi-intracellular niche ayaziwa.Apha, siye saphanda iindlela eziphambili ze-C metabolism ezisetyenziswa nguBrucella abortus ngokucima i-puative fructose-1,6-bisphosphatase (fbp kunye ne-glpX), i-phosphoenolpyruvate carboxykinase (pckA), i-pyruvate phosphate dikinase (ppdK), kunye ne-malic enzyme (mae) imfuza.Kwi-gluconeogenic kodwa hayi kwimithombo yeendaba etyebileyo, ukukhula kwe-ΔppdK kunye ne-Δmae eziguquguqukayo zonakaliseka kakhulu kwaye ukukhula kwe-Δfbp-ΔglpX eguquguqukayo kabini kwancitshiswa.Kwi-macrophages, kuphela i-ΔppdK kunye ne-Δmae mutants ibonise ukunciphisa ukuphindaphinda, kunye nezifundo kunye ne-ΔppdK i-mutant iqinisekisile ukuba ifikelele kwi-niche ephindaphindayo.Ngokufanayo, kuphela i-ΔppdK kunye ne-Δmae mutants yancitshiswa kwiimpuku, yangaphambili yacocwa ngeveki ye-10 kwaye yokugqibela iqhubeka ixesha elide kuneeveki ze-12.Siphinde saphanda umjikelo we-glyoxylate.Nangona i-aceA (isocitrate lyase) umsebenzi womkhuthazi waphuculwa kwindawo etyebileyo, ukuphazamiseka kwe-aceA kwakungekho mpembelelo kwi-vitro okanye ekuphindaphindeni kwi-macrophages okanye kwi-spleen ye-mouse.Iziphumo zibonisa ukuba i-B. abortus ikhula nge-intracellularly isebenzisa ukunikezelwa okulinganiselwe kwe-6-C (kunye ne-5-C) ishukela ehlawulwa yi-glutamate kwaye mhlawumbi nezinye i-amino acids ezingena kumjikelezo we-Krebs ngaphandle kwendima ebalulekileyo ye-glyoxylate shunt.