I-DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) CAS: 28718-90-3 99% Umgubo ophuzi
| Inombolo Yekhathalogu | XD90505 |
| Igama lemveliso | I-DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) |
| CAS | 28718-90-3 |
| Ifomula yeemolekyuli | C16H15N5 · 2HCl |
| Ubunzima beMolekyuli | 350.25 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 2933998090 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo otyheli |
| Isivavanyi | 99% imizuzu |
| Isetyenziselwa uphando kuphela, hayi ukusetyenziswa komntu | Uphando lusetyenziselwa kuphela, kungekhona ukusetyenziswa kwabantu |
I-Melatonin ibandakanyeka kwimisebenzi emininzi yomzimba, kwaye ineziphumo ezahlukileyo kwi-apoptosis kwiiseli eziqhelekileyo kunye nomhlaza.Nangona kunjalo, indlela yeendima zayo ze-antitumor ayiqondwa kakuhle.Kolu phononongo, sibonisa ukuba i-melatonin yonyusa i-apoptosis eyenziwe yi-tunicamycin kwi-carcinoma yebele yomntu i-MDA-MB-231 iiseli.I-Melatonin iphakamisa-ilawula iprotheyini ye-pro-apoptotic i-Bim expression kwi-transcriptional level phambi kwe-tunicamycin.I-Melatonin inqanda i-tunicamycin-induced expression ye-COX-2 kwiiseli ze-MDA-MB-231.Ngaphezu koko, ukuvinjelwa komsebenzi we-COX-2 usebenzisa i-COX-2 inhibitor, i-NS398, kwandisa i-apoptosis ye-tunicamycin.Okubangel 'umdla kukuba, ezi ziphumo azizange zidibaniswe neendlela zesignali ye-melatonin receptor.I-Pertussis toxin (i-general Gi protein inhibitor) okanye i-luzindole (i-melatonin receptor antagonist engabonakaliyo) ayizange iguqule umphumo we-melatonin.Ukongezelela, i-melatonin ivalwe i-tunicamycin-induced i-NF-κB umsebenzi wokubhaliweyo, i-p65 i-nuclear translocation, kunye ne-p38 MAPK act ivation.I-Melatonin-mediated p38 MAPK inhibition ibe negalelo ekunciphiseni uzinzo lwe-COX-2 mRNA.Kuthatyathwe kunye, iziphumo zethu zibonisa ukuba i-melatonin iphucula umsebenzi we-antitumor ngokunyusa-ulawulo lwe-Bim expression kunye nolawulo oluphantsi lwe-COX-2 intetho kwiiseli ze-MDA-MB-231 eziphathwa nge-tunicamycin.
