Iimveliso

Siphuhlise inoveli encinci ye-RNA (i-siRNA) ephazamisayo inkqubo yokuhanjiswa usebenzisa i-ternary complex ene-polyethyleneimine (PEI) kunye ne-γ-polyglutamic acid (γ-PGA), ebonisa umphumo wokuthulisa kwaye akukho cytotoxicity.I-binary complexes ye-siRNA ene-PEI yayimalunga ne-73-102 nm ngobukhulu be-particle kunye ne-45-52 mV kwi-ζ-enokwenzeka.Umphumo wokuthulisa we-siRNA / PEI complexes wanda ngokunyuka kwe-PEI, kunye ne-siRNA / PEI complexes ezinomlinganiselo wentlawulo enkulu kune-16 ibonise i-luciferase ephawulekayo kwi-mouse colon carcinoma cell line ebonisa rhoqo i-luciferase (i-Colon26 / iiseli ze-Luc).Nangona kunjalo, i-cytotoxicity enamandla kunye ne-agglutination yegazi yabonwa kwi-siRNA / Lipofectamine complex kunye ne-siRNA / PEI16.Ukutshaja kwakhona iikhompawundi ze-cationic kunye nekhompawundi ye-anionic kwaxelwa ukuba yindlela ethembisayo yokoyisa ezi tyhefu.Ngoko ke silungiselele ii-ternary complexes ze-siRNA kunye ne-PEI (i-charge ratio 16) ngokongezwa kwe-γ-PGA ukunciphisa i-cytotoxicity kunye nokuhambisa i-siRNA.Njengoko kulindelekile, i-cytotoxicity ye-ternary complexes yehla ngokunyuka komxholo we-γ-PGA, oye wanciphisa i-ζ-amandla eekhompleksi.Isiphumo esinamandla sokuthulisa esithelekiseka ne-siRNA/Lipofectamine complex yafunyanwa kwii-ternary complexes eziquka i-γ-PGA ene-anionic surface charge.Ukubandakanywa okuphezulu kwee-ternary complexes kwiiseli ze-Colon26 / Luc zaqinisekiswa nge-fluorescence microcopy.Emva kokuba kuphunyezwe i-exogenous yejini eyosulelwe ngaphandle, ukukwazi kwe-complex ukulamla i-endogenous yejini yokugcina indlu, i-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), yavavanywa kwiiseli ze-B16-F10.I-ternary complex (i-siRNA/PEI16/γ-PGA12 complex) ibonise isiphumo esibalulekileyo se-GAPDH.Ke, siphuhlise inkqubo yokuhanjiswa kwe-siRNA eluncedo.