I-GSH Cas: 70-18-8 98% I-crystalline powder emhlophe
| Inombolo Yekhathalogu | XD90227 |
| Igama lemveliso | GSH |
| CAS | 70-18-8 |
| Ifomula yeemolekyuli | I-C10H17N3O6S |
| Ubunzima beMolekyuli | 307.323 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 29309098 |
Ukucaciswa kweMveliso
| Ukucaciswa kweMveliso | |
| Indawo yokunyibilika | 185°C |
| IBanga | Pharma iBanga |
| Iintsimbi ezinzima | 10ppm ubukhulu |
| Arsenic | 2ppm ubukhulu |
| Ukuchongwa | IR : Iyafana ne-Reference Spectrum |
| Ilahleko ekomisweni | 0.5% ubukhulu |
| Ubushushu boGcino | +20 °C |
| Ubunzima beMolekyuli | 612.63 |
| Isivavanyi | 98.0% min |
| Ukujikeleziswa kwe-Optical | -15.5 ° - -17.5 deg |
| Intsalela kwi-Ignition | 0.1% ubukhulu |
| Ukucaca kwesisombululo | Icacile kwaye ayinambala |
| Imbonakalo | Umgubo omhlophe wekristale |
| Into enxulumeneyo | Iyonke: 2.0% max, GSSG:1.5% max |
Iprotheyini ye-ORF45 ye-Kaposi's sarcoma-associated herpesvirus (KSHV) yi-gammaherpesvirus-specific immediate-early tegument protein.Uphononongo lwethu lwangaphambili luveze indima yalo ebalulekileyo kumanqanaba okuqala kunye nasemva kwexesha losulelo lwe-KSHV.Kolu phononongo, siphonononge i-interantome ye-ORF45 sisebenzisa ipaneli ye-monoclonal antibodies.Ukongeza kwiiprotheni ezichongiweyo ze-extracellular regulated kinase (ERK) kunye ne-p90 ribosomal S6 kinase (RSK), sifumene ezinye iiprotheni ezininzi ezidibeneyo, ezibandakanya ezibalaseleyo ze-~38 kDa kunye ne-130 kDa.I-mass spectrometry ibonise ukuba iprotheni ye-38-kDa yintsholongwane ye-ORF33 kunye neprotheni ye-130-kDa yi-USP7 yeselula (ubiquitin-specific protease 7).Senze imephu ye-ORF33-binding domain kwi-carboxyl-terminal 19 amino acids (aa) egcinwe kakhulu ye-ORF45 kunye ne-USP7-binding domain kwi-motif yesivumelwano esixeliweyo kumbindi wengingqi ye-ORF45.Ngokusebenzisa i-immunofluorescence staining, siye saqwalasela i-colocalization ye-ORF45 kunye ne-ORF33 okanye i-USP7 zombini phantsi kwee-ion ze-condit ezosulelekileyo kunye neeseli ezosulelwe yi-KSHV.Ngaphezu koko, siqaphele ukutshintshwa kwe-ORF45 exhomekeke kwinxalenye ye-ORF33/USP7 ukusuka kwi-nucleus ukuya kwi-cytoplasm.Sifumene ukuba i-ORF45 ibangele ukwanda kwe-ORF33 yeprotheyini yokuqokelela yapheliswa ukuba mhlawumbi i-ORF33- okanye i-USP7-binding domain kwi-ORF45 yacinywa.Ngapha koko, ukucinywa kwe-carboxyl terminus ye-ORF45 kwi-genome ye-KSHV kunciphise kakhulu inqanaba leprotein ye-ORF33 kwiiseli ezosulelwe yi-KSHV kunye nokuphelisa ukuveliswa kwee-virions zenzala.Ngokudibeneyo, iziphumo zethu azivezi kuphela amacandelo amatsha e-ORF45 interactome, kodwa zikwabonisa ukuba intsebenziswano phakathi kwezi proteni ibalulekile kwi-KSHV lytic replication.I-Kaposi's sarcoma-associated herpesvirus (KSHV) yi-causative agent yee-cancer ezininzi zabantu.I-KSHV ORF45 yiprotheni esebenzayo efunekayo kwi-KSHV lytic replication, kodwa iindlela ezichanekileyo i-ORF45 eyenza ngayo imisebenzi yayo ebalulekileyo ayicacanga.Uphononongo lwethu lwangaphambili lubonise ukuba yonke i-ORF45 pr otein kwiiseli ikhona kwi-high-molecular-weight complex complexes.Ke ngoko siye sazama ukubonisa unxibelelwano lwe-ORF45 ukubonelela ngengqiqo kwiindima zayo ngexesha lokuphindaphinda kwe-lytic.Ukusebenzisa ipaneli ye-antibodies ye-monoclonal, sihlolisise i-ORF45 interactome kwiiseli ezosulelwe yi-KSHV.Sichonge amaqabane amabini amatsha abophezelayo e-ORF45: iprotheni yentsholongwane ye-ORF33 kunye ne-cellular ubiquitin-specific protease 7 (USP7).Sibonisa ngakumbi ukuba intsebenziswano phakathi kwe-ORF45 kunye ne-ORF33 ibalulekile kwimveliso esebenzayo yeentsholongwane ze-KSHV zentsholongwane, ebonisa ukuba ukuphazamiseka okujoliswe kuyo kolu nxibelelwano kunokubonisa isicwangciso senoveli ukuvimbela ukuphindaphinda kwe-KSHV lytic.
