I-Hygromycin B CAS: 31282-04-9 Buff powder
| Inombolo Yekhathalogu | XD90374 |
| Igama lemveliso | Hygromycin B |
| CAS | 31282-04-9 |
| Ifomula yeemolekyuli | I-C20H37N3O13 |
| Ubunzima beMolekyuli | 527.52 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 2941900000 |
Ukucaciswa kweMveliso
| Iintsimbi ezinzima | 20mg/kg ubuninzi |
| pH | 7-9,5 |
| Isivavanyi | 99% |
| Ilahleko ekomisweni | 5% max |
| umsebenzi | 950u/mg min |
| Ammonium | 1% max |
| Uthuthu olufakwe emanzini | 5% max |
| Imbonakalo | Umgubo weBuff |
| Purity TLC | >90% |
Ukuguga ngokulandelelana kweeseli zegwele ngokuqhelekileyo kusetyenziswa njengemodeli yokuguga kweeseli ze-post-mitotic.Igwele iSaccharomyces cerevisiae ekhule kwiglucose kubukho beammonium sulphate isetyenziswa ikakhulu kuphando lokuguga kweyeast.Siye sahlalutya ukuguga ngokulandelelana kwegwele le-Hansenula polymorpha ekhule kwiimeko ezifuna i-peroxisome metabolism yokukhula. Ubomi bexesha le-H. polymorpha buphuculwa kakhulu xa iiseli zikhuliswe kwi-methanol okanye i-ethanol, i-metabolized yi-enzymes peroxisome, ngokumalunga nokukhula kwi-glucose. loo nto ayifuni peroxisomes.Ubomi obufutshane be-H. polymorpha kwi-glucose ikakhulu ngenxa ye-asidi ephakathi, kanti i-ROS inokwenzeka ukuba ayidlali indima ebalulekileyo.Ukukhula kweeseli kwi-methanol/methylamine endaweni ye-methanol/ammonium sulphate kubangele ukongezwa kwexesha lokuphila.Oku kwakungahambelani neasidi ephakathi.Sibonisa ukuba i-oxidation ye-methylamine nge-peroxisomal amine oxidase kwiimeko zendlala yekhabhoni inoxanduva lokwandiswa kwexesha lokuphila.Imveliso ye-methylamine oxidation ye-formaldehyde iphinda ifakwe i-oxidized ekhokelela ekuveliseni i-NADH, enegalelo ekwandiseni isizukulwana se-ATP kunye nokunciphisa amanqanaba e-ROS kwinqanaba elimileyo.Ngaphezu koko, ukubanakho ukuvelisa i-NADH kwiimeko zokulamba kwekhabhoni ngumthombo wenitrogen ophilayo uxhasa ukwandiswa kwexesha lokuphila kweseli.Ngenxa yoko, ukutolikwa kohlalutyo lwe-CLS kwigwele kufuneka kubandakanye iziphumo ezinokubakho kwimeko yamandla yeseli.
