L-Lactate dehydrogenase CAS: 9001-60-9
| Inombolo Yekhathalogu | XD90395 |
| Igama lemveliso | L-Lactate dehydrogenase |
| CAS | 9001-60-9 |
| Ifomula yeemolekyuli | - |
| Ubunzima beMolekyuli | - |
| IKhowudi yoMrhumo eHarmonized | 35079090 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo omhlophe |
Uhlalutyo lweziganeko ze-proteome-wide phosphorylation zisengumngeni omkhulu wohlalutyo ngenxa yobunzima obukhulu beenethiwekhi ze-protein phosphorylation.Kulo msebenzi, sivavanya ukuhambelana kweLys-N, iLys-C, kunye ne-trypsin malunga nokukwazi kwabo ukufaka isandla kuhlalutyo lwehlabathi lwe-phosphoproteome.Uguqulelo olusulungekileyo lwe-low-pH elomeleleyo lotshintshiselwano lwe-cation lusetyenziselwe ukwahlula ngokufanelekileyo i-N-terminally acetylated, phosphorylated, kunye neepeptides ezingaguqukiyo.I-5036 epheleleyo ye-phosphopeptides engafunekiyo inokuchongwa ngezinga lokufumanisa ubuxoki <1% ukusuka kwi-1 mg yeprotheni usebenzisa ukudibanisa kwee-enzyme ezintathu.Idatha yethu ibonise ukuba ukugqithwa phakathi kweeseti zedatha ye-phosphopeptide eyenziwe ngeeproteases ezahlukeneyo yayingaphantsi, kanti ukugqithwa phakathi kweeseti zedatha ye-tryptic eveliswa ngokufanayo kufunyenwe ukuba ubuncinane ngamaxesha e-4 aphezulu.Ngale ndlela, usetyenziso olunxuseneyo lwe-Lys-N kunye ne-trypsin lwenza ukuba i-72% yonyuke kwinani le-phosphopeptides echongiweyo njengoko ithelekisa i-d ne-trypsin iyodwa, ngelixa uvavanyo lokuphindaphinda lwe-trypsin lukhokelele kunyuso lwama-25%.Ke, xa sigxile kuphela kwidatha ye-trypsin kunye neLys-N, sichonge iiphosphopeptides ezingama-4671 ezingafunekiyo.Uhlalutyo olongezelelweyo lweendawo ezichongiweyo lubonise ukuba i-Lys-N kunye neeseti zedatha ye-trypsin ziye zatyetyiswa kwiimotifs ze-phosphorylation ezahlukeneyo, ngakumbi ubungqina bokuba iindlela ze-multiprotease zixabiseke kakhulu kuhlalutyo lwe-phosphoproteome.
