Iproteinase K ye-albhamu ye-tritirachium Cas: 39450-01-6 99% Umgubo omhlophe
| Inombolo Yekhathalogu | XD90434 |
| Igama lemveliso | Iproteinase K ye-albhamu ye-tritirachium |
| CAS | 39450-01-6 |
| Ifomula yeemolekyuli | I-C29H27N2O12P |
| Ubunzima beMolekyuli | 626.50468 |
| Iinkcukacha zokuGcina | -15 ukuya -20 °C |
| IKhowudi yoMrhumo eHarmonized | 35079090 |
Ukucaciswa kweMveliso
| Ubunzima obuthile | I-40un / mg iprotheni min |
| Umsebenzi we-Enzyme | 30un / mg min ubunzima owomileyo |
| Imbonakalo | Umgubo omhlophe |
| Isivavanyi | ≥99% |
Izifundo ze-genome-wide chromatin immunoprecipitation (ChIP) ziye zazisa ingqiqo ebalulekileyo kwi-genomic yendawo yeeprotheni ezinxulumene ne-chromatin kunye nokuguqulwa kwe-histone.Isixa esikhulu sedatha eyenziwe ngolu hlalutyo, nangona kunjalo, lufuna iindlela ezivumela ukuqinisekiswa ngokukhawuleza kunye nohlalutyo lokubaluleka kwezinto eziphilayo.Ngaphezu koko, kusekho iiprotheyini kunye neethagethi zokuguqulwa ekunzima ukuzibona usebenzisa iindlela eziqhelekileyo ze-ChIP.Ukujongana nale miba, siphuhlise inkqubo ye-chromatin immunoprecipitation ekhawulezileyo esiyibiza ngokuba yiZipChip.I-ZipChip inciphisa kakhulu ixesha kwaye ikhulise uvakalelo oluvumela ukukhangela ngokukhawuleza kwe-loci ezininzi.Apha sichaza indlela i-ZipChIP eyenza ngayo ukufumanisa ukuguqulwa kwe-histone (i-H3K4 i-mono- kunye ne-trimethylation) kunye ne-yeast histone demethylases, i-Jhd2 kunye ne-Rph1, eyayinzima ukuyifumanisa ngokusebenzisa iindlela eziqhelekileyo.Ngaphezu koko, sibonisa ukuguquguquka kwe-ZipChIP ngokuhlalutya ukutyebisa kwe-histone deacet ylase Sir2 kwi-heterochromatin kwigwele kunye nokutyebisa kwe-chromatin remodeler, i-PICKLE, kwi-euchromatin kwi-Arabidopsis thaliana.
