Iimveliso

Ukuhlukana kwee-fibroblasts ze-orbital zibe yi-adipocytes ekhulile kunye nokuqokelelwa okulandelayo kwezicubu ze-adipose kuboniswe kwinkqubela phambili ye-Graves 'orbitopathy (GO).I-Autophagy ibandakanyeka kwi-adipogenesis, kodwa incinci iyaziwa malunga nendima ye-autophagy ekuqaliseni nasekuqhubeleni phambili kwe-GO.Injongo yolu phononongo kukuphanda indima ye-autophagy kwi-pathogenesis ye-GO.Orbital adipose / i-connective tissue explants evela kwizigulane ezine-GO kunye nezifundo eziqhelekileyo, kunye ne-fibroblasts eyodwa ye-orbital, yahlaziywa.I-Adipogenesis yenziwa kusetyenziswa ukwahlula okuphakathi kunye okanye ngaphandle kwe-hydrogen peroxide, kwaye i-autophagy yasetyenziswa kusetyenziswa i-bafilomycin A1 kunye ne-Atg5 ejoliswe kwi-hairpin emfutshane ye-RNA (shRNA).Ii-autophagosomes zachongwa nge-electron microscopy.Ukubonakaliswa kofuzo olunxulumene ne-autophagy kunye nezinto ezikhutshelweyo ezinxulumene ne-adipogenesis zahlalutywa yi-real time reverse transcription-polymerase chain reaction kunye / okanye uhlalutyo lwe-Western blot.I-Lipid droplet accumulation ihlolwe yi-Oil Red O staining.I-autophagic vacuoles yayininzi kakhulu kwiiseli ze-GO kune-non-GO cells (p <0.05).Ukubonakaliswa kweejene ezinxulumene ne-autophagy kwakuphezulu kakhulu kwi-GO tissues kunye neeseli kunezo zingekho kwi-GO, ngokulandelanayo.I-Interleukin-1β yanda i-LC3-II, i-p62, kunye ne-Atg7 iprotheni kwiiseli ze-GO.Ukuqokelela kwe-Autophagosome kuboniswe ngosuku lwe-4 ye-adipogenesis kwaye yehla nge-10, kunye nokuqulunqwa kwe-lipid droplet.Ukubonakaliswa kwe-LC3 kunye neeprotheni ze-p62 zanda kwiiyure ze-48 zokwahlula kwaye ziyancipha ngokuthe ngcembe ukusuka kwi-4 ukuya kwi-10. Unyango lwe-Bafilomycin A1 kunye ne-Atg5 knockdown yi-shRNA inhibited lipid droplet accumulation kunye ne-pressure expression of markers adipogenic.I-autophagy yanda kwi-GO tissue kunye neeseli xa kuthelekiswa no I-non-GO izicubu kunye neeseli, ezicebisa ukuba i-autophagy idlala indima kwi-GO pathogenesis.Ukunyangwa kwe-autophagy kunokuba yinjongo yonyango ye-GO.