I-Streptomycin sulfate CAS: 3810-74-0 Umgubo omhlophe
| Inombolo Yekhathalogu | XD90361 |
| Igama lemveliso | Streptomycin sulfate |
| CAS | 3810-74-0 |
| Ifomula yeemolekyuli | (C21H39N7O12)2·3H2SO4 |
| Ubunzima beMolekyuli | 1457.39 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 29412080 |
Ukucaciswa kweMveliso
| IBanga | USP36/EP8.0 |
| pH | 4.5-7.0 |
| Ilahleko ekomisweni | ≤7.0% |
| Isivavanyi | ≥720IU/MG |
| Sulfate | 18.0 ~ 21.5% |
| Imbonakalo | Umgubo omhlophe okanye ophantse ube mhlophe, unyibilika kakhulu emanzini, phantse awunyibiliki kwi-ethanol. |
| IMethanol | ≥0.3% |
| Streptomycin B | ≤3.0% |
| Uvavanyo lombala | ≥90.0% |
| Iasidi yeSulfric | ≤1.0% |
| Umxholo we-sodium sulphite egxininisiweyo | ≤0.4% |
Nangona i-etiology ye-intervertebral disc degeneration ingaqondwa kakuhle, enye indlela yokuthintela le nkqubo inokuthintela i-apoptosis.Kuphononongo lwangoku, iziphumo ezichasene ne-apoptotic ye-carboxymethylated chitosan (CMCS) kwiiseli ze-nucleus pulposus (NP) zaphandwa ngenjongo yokuphucula ukusinda kweeseli zediski.Iiseli ze-Rat NP zaye zodwa kwaye zakhuliswa kwi-vitro, kwaye i-hydrogen peroxide (H2O2) yayisetyenziselwa ukwakha imodeli ye-NP yeseli ye-apoptosis.Ukusebenza kweeseli kuye kwavavanywa ngekiti yokubala kweeseli-8.Umlinganiselo weeseli ze-apoptotic uhlolwe yi-annexin V-fluorescein isothiocyanate (FITC) kunye nepropidium iodide (PI) uhlalutyo oluphindwe kabini lwe-staining, kwaye i-morphology yabonwa ngu-Hoechst 33342 staining.I-mitochondrial membrane enokubakho yeeseli ze-NP yavavanywa yi-rhodamine 123 fluorescence staining.Reverse transcription (RT)‑quantitative polymerase chain reaction (qPCR) yenziwa ukulinganisa amanqanaba e-mRNA ye-inducible nitric oxide synthase (iNOS), caspase-3, B-cell lymphoma (Bcl)‑2, uhlobo lwe-II collagen kunye ne-aggrecan.Uhlalutyo lwe-blot lwaseNtshona lwenziwa ukufumana amanqanaba eprotheyini ye-iNOS kunye ne-Bcl-2.I-annexin V‑FITC/PI kunye ne-Hoechst 33342 iziphumo zokungcolisa zibonise ukuba i-CMCS ikwazile ukukhusela iiseli ze-NP kwi-apoptosis ngendlela exhomekeke kwidosi.I-Rhodamine 123 staining icacise ukuba i-CMCS inciphise ukonakala kwenwebu ye-mitochondrial enokubakho kwiiseli ze-NP eziphathwa nge-H2O2.Ukunciphisa i-caspase-3 kunye nokunyuka komsebenzi we-Bcl-2 kwafunyanwa kwiiseli ze-NP eziphathwa nge-CMCS yi-RT-qPCR kunye nohlalutyo lwe-blot yasentshona.I-CMCS iphinde yakhuthaza ukwanda kunye nokukhuselwa kohlobo lwe-collagen ye-II kunye ne-aggrecan kwiiseli ze-NP eziphathwa nge-H2O2.I-CMCS ibonakaliswe ukuba isebenze ekukhuseleni i-apoptotic cell death in vitro, ebonisa iingenelo ezinokuthi zisebenzise le ndlela yokwelapha ekulawuleni ukuchithwa kwe-disc.
