Thrombin CAS:9002-04-4
| Inombolo Yekhathalogu | XD90400 |
| Igama lemveliso | Thrombin |
| CAS | 9002-04-4 |
| Ifomula yeemolekyuli | C6H14O6 |
| Ubunzima beMolekyuli | 182.17 |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 30021900 |
Ukucaciswa kweMveliso
| Indawo yokunyibilika | 166-168 deg C |
| Imbonakalo | Umgubo omhlophe |
Izinzwa ze-PH zenziwe ngefilimu ye-aridium oxide ye-anodically electrodepositing iridium oxide (AEIROFs) kwii-microelectrodes kwiitships zaza zagqunywa nge-poly(ethyleneimine) (PEI) yozinzo lomatshini.Ezi zibonisa impendulo ye-super-Nernstian kwi-pH ukusuka kwi-pH 4.0 ukuya kwi-7.7 kwi-chloride-free phosphate buffer.Umphezulu we-chip wawugqunywe nge-fibronectin yokufakelwa kweeseli ze-porcine aortic endothelial (PAECs).Isakhono sokusebenza senzwa ye-pH yokubeka iliso kwiinguqu ezibukhali ze-pH zendawo zaphandwa ngokuvuselela ii-PAEC nge-thrombin.Iziphumo zethu zibonisa ukuba i-thrombin yenza i-acute extracellular acidification ye-PAECs kunye nokuchithwa kwe-fibronectin, okubangela ukuba i-pH yendawo yehle.Ukusetyenziswa kwe-PD98059, i-mitogen-activated protein kinase (MAPK) inhibitor, ukunciphisa i-extracellular acidification kunye nokunyuka kwe-pH yendawo yabonwa.Olu pho nonongo lubonisa ukuba i-pH sensors zethu zinokuququzelela uphando lweempendulo zeselula ezibukhali ekukhuthazeni ngokubeka iliso kwixesha langempela, utshintsho lwe-pH yendawo yeeseli ezifakwe kwiinzwa.
