Tris maleate Cas: 72200-76-1 99.5% White crystalline powder
Inombolo Yekhathalogu | XD90057 |
Igama lemveliso | Tris maleate |
CAS | 72200-76-1 |
Ifomula yeemolekyuli | C4H11NO3 · C4H4O4 |
Ubunzima beMolekyuli | 237.21 |
Iinkcukacha zokuGcina | I-Ambient |
IKhowudi yoMrhumo eHarmonized | 29221985 |
Ukucaciswa kweMveliso
Imbonakalo | Umgubo omhlophe wekristale |
Isivavanyi | ≥ 99.5% |
I-Cocrystallization ye-lysyl-tRNA synthetase esuka kwi-Thermus thermophilus kunye ne-tRNAlys yayo kunye ne-Escherichia coli tRNAlys.
I-Lysyl-tRNA synthetase esuka kwi-Thermus thermophilus iye yadityaniswa nokuba yi-tRNAlys yayo yonxulumano okanye i-Escherichia coli tRNAlys isebenzisa i-ammonium sulfate njenge-precipitant.Iikristale zikhula kwizisombululo eziqulethe i-1: 2.5 stoichiometry ye-synthetase dimer ukuya kwi-tRNA kwi-18-22% ye-ammonium sulfate kwi-50 mM Tris-maleate buffer kwi-pH 7.5.Zombini ii-complexes zenza i-square prismatic, i-tetragonal crystals ezineemitha zeeseli zeyunithi ezifanayo kakhulu (a = b = 233 A, c = 119 A) kunye ne-diffract ubuncinane kwi-2.7 A isisombululo.Nangona kunjalo i-homocomplex yeyeqela lesithuba P42(1)2 kunye ne-heterocomplex yeqela lesithuba i-I422.