Uridine,2′-deoxy-5-ethynyl- Cas:61135-33-9
| Inombolo Yekhathalogu | XD90589 |
| Igama lemveliso | Uridine,2'-deoxy-5-ethynyl- |
| CAS | 61135-33-9 |
| Ifomula yeemolekyuli | I-C11H12N2O5 |
| Ubunzima beMolekyuli | 252.23 |
| Iinkcukacha zokuGcina | -20°C |
| IKhowudi yoMrhumo eHarmonized | 29349990 |
Ukucaciswa kweMveliso
| Imbonakalo | Mhlophe ukuya kumgubo oLuhlaza ukuya kwikristale |
| Isivavanyi | ≥99% |
Olu phononongo lwenziwe ukuphanda isiphumo sokwanda kweepeptide zesoya yemifuno kwiiseli ze-stem yabantu abadala (ASCs) ngokungabikho kwe-serum kunye neendlela zabo zokwenza.Ukwandiswa kweeseli ze-mesenchymal stem cell (ADSCs) kunye neentambo ze-mesenchymal stem cell (CB-MSCs) eziphathwe nge-soy peptides zifunyenwe zanda kakhulu kwi-3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide kunye ne-Click-iT 5-ethynyl-2'-deoxyuridine flow cytometry assay.Ukongeza, i-soy peptides ikhokelele kwi-stepwise phosphorylation ye-p44/42 MAPK (ERK), i-mammalian target of rapamycin (mTOR), i-p70 S6 kinase, i-S6 ribosomal protein (S6RP) kunye ne-eukaryotic initiation factor 4E (eIF4E) kwii-ADSCs.Ngaphezu koko, uhlalutyo lobungakanani be-cytokines lubonise ukuba ukuveliswa kokuguqulwa kwe-factor factor-beta1 (TGF-β1), i-vascular endothelial growth factor kunye ne-interleukin-6 yanda kakhulu ekuphenduleni unyango kunye ne-soy peptides kuzo zombini ii-ADSC kunye ne-CB-MSCs.Ngokufanayo, i-soy peptide-induced phosphorylation ye-ERK/mTOR/S6RP/eIF4E indlela yayivaliwe ekuphenduleni unyango lwangaphambili kunye ne-PD98059, i-ERK inhibitor ethile.Ngaphezu koko, ukuvinjelwa kwe-TGF-β1 ngokusebenzisa i-PD98059 yonyango kunye nokuncipha okulandelelanayo kwi-ADSC yokwanda kwabonisa ukuba i-TGF-β1 ibangela i-phosphorylation ye-mTOR/S6RP/eIF4E.Ngokudibeneyo, iziphumo zolu phononongo zibonisa ukuba imveliso exhomekeke ku-ERK ye-TGF-β1 idlala indima ebalulekileyo kwi-soy peptide-induced proliferation of ADSCs under serum-free conditions.Copyright © 2012 Elsevier Inc. Onke amalungelo agciniwe.
