Iimveliso

Izaleko zebrashi yePolymer esekwe kwibloko yecopolymers yepoly(oligo(ethylene glycol) methacrylate) (POEGMA) kunye nepoly(glycidyl methacrylate) (PGMA) zenziwa kwi-silicon wafers yi activators eveliswe yi-electron transfer atom transfer radical polymerization (AGET ATRP).Iindidi ezahlukeneyo ze-biomolecule zinokudityaniswa kolu lwaleko lwebrashi ngokusabela kwamaqela e-PGMA epoxide kunye namaqela e-amino kwi-biomolecule, ngelixa i-POEGMA, exhathisa i-adsorption yeprotheyini engachazwanga, ibonelela ngendawo echasayo.Imiphezulu ibonakaliswe nge-engile yokudibana kwamanzi, i-ellipsometry, kunye ne-Fourier transform infrared spectroscopy (FTIR) ukuqinisekisa ukusabela kohlengahlengiso.Ukwahlulwa kwesigaba seebhloko ze-copolymer kwiileyile zabonwa yi-AFM.Isiphumo seempawu ezingaphezulu kwi-protein conjugation yaphandwa kusetyenziswa iindlela ze-radiolabeling.Kwaboniswa ukuba imiphezulu enemigangatho ye-POEGMA yayixhathisa iprotheyini, ngelixa ubuninzi beprotheyini edityaniswe kwindawo eguquliweyo ye-diblock copolymer eyonyuswa ngokunyuka kobunzima be-PGMA.Umsebenzi we-lysozyme odityaniswe phezu komphezulu nawo unokulawulwa ngokuguquguquka kobunzima bomaleko we-copolymer.Xa i-biotin idityaniswe kwibhloko ye-copolymer grafts, umphezulu wawuhlala uxhathisa kwi-adsorption yeprotheyini engabonakaliyo kodwa wabonisa ukubopha okuthe ngqo kwe-avidin.Ezi zakhiwo, oko kukuthi, ubuninzi obulawulwa kakuhle kunye nomsebenzi we-biomolecules edibeneyo kunye neenkcukacha zokusebenzisana kunye nee-biomolecules ezijoliswe kuzo zingasetyenziselwa ukuphuculwa komlinganiselo wesignali-kwingxolo kwizicelo zenzwa.Ngokubanzi, umphezulu onjalo unokuba luncedo njengezinto eziqatshelweyo zebhayoloji ezithe ngqo kwizinto ezisebenza ngebhayoloji.