Iimveliso

Isiphumo se-acidosis, uphawu lwe-biochemical ye-tumor microenvironment, kwinkqubela phambili yomhlaza kunye ne-metastasis inzima.Zombini iziphumo ze-pro- kunye ne-anti-tumorigenic ye-acidosis ziye zaxelwa kwaye i-acidic microenvironment iye yasetyenziselwa ukuhanjiswa okuthe ngqo kwamachiza, ii-agent ze-imaging, kunye ne-genetic constructs zibe ngamathumba.Kolu phononongo siphanda ukusasazeka kunye nokunamathela kwi-focal ye-B16F10 yeeseli ze-melanoma ezenziwe ngofuzo ukuze zigqithise i-pH-sensing G protein-coupled receptor GPR4.Ngokusebenzisa i-cell attachment assays sifumene ukuba i-GPR4 overexpression ilibazise ukusasazeka kweseli kwaye yatshintsha indawo yendawo ye-dynamic focal adhesion complex, efana ne-localization of phosphorylated focal adhesion kinase (FAK) kunye ne-paxillin, kwi-acidic pH.Iprotheyini ye-G enokubakho kunye neendlela ezisezantsi zomqondiso ezinoxanduva lwezi ziphumo nazo zaphandwa.Ngokusebenzisa i-Rho inhibitor CT04 (C3 transferase), i-Rho-associated kinase (ROCK) inhibitors Y27632 kunye ne-thiazovivin, i-myosin light chain kinase (MLCK) inhibitor staurosporine okanye i-G12/13 yokwakhiwa kwe-inhibitory, ukusasazeka kweeseli kwabuyiselwa kwaye ukusebenza kweendlela ze-Gq kunye ne-Gs kunesiphumo esincinci okanye akukho nto.Zizonke iziphumo zethu zibonisa ukuba nge-G12/13/Rho indlela yokubonisa i-GPR4 imodareyitha i-focal adhesion dynamics kunye nokunciphisa ukusasazeka kweeseli kunye ne-membrane ruffling.