I-Cytidine Cas: 65-46-3 I-crystalline powder emhlophe 99%
| Inombolo Yekhathalogu | XD90586 |
| Igama lemveliso | Cytidine |
| CAS | 65-46-3 |
| Ifomula yeemolekyuli | I-C9H13N3O5 |
| Ubunzima beMolekyuli | 243.22 |
| Iinkcukacha zokuGcina | I-Ambient |
| IKhowudi yoMrhumo eHarmonized | 29349990 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo omhlophe wekristale |
| Isivavanyi | 99% |
| Iintsimbi ezinzima | <20ppm |
| Ifomula yeMichiza | I-C9H13N3O5 |
| Ilahleko ekomisweni | <0.5 % |
| Intsalela kwi-Ignition | <0.10 % |
Ubonelelo ngeselfowuni ye-deoxynucleoside triphosphates (dNTPs) lubalulekile kuphindaphindo lwe-DNA kunye nokulungiswa.Kule sifundo, siye saphanda indima ye-CMP / UMP kinase (CMPK), i-enzyme ivuselela ukubunjwa kwe-CDP, ekulungisweni kwe-DNA.I-Knockdown ye-CMPK ilibazisa ukulungiswa kwe-DNA ngexesha lokubuyiselwa kumonakalo we-UV kwiiseli ezinqatshelwe kwi-serum kodwa kungekhona kwiiseli ngaphandle kokungabikho kwe-serum.Ukunikezelwa kwangaphandle kwe-cytidine okanye i-deoxycytidine iququzelela ukulungiswa kwe-DNA exhomekeke kwi-CMPK kwiiseli ze-serum-deprived cell, ebonisa ukuba i-synthesis ye-dCDP okanye i-CDP inquma izinga lokulungiswa.Nangona kunjalo, i-CMPK knockdown ayichaphazeli umgangatho ozinzileyo we-dCTP kwiiseli ze-serum-deprived.Emva koko sifumene indawo ye-CMPK kwiindawo zomonakalo we-DNA kunye nokwakheka kwayo okuyinkimbinkimbi kunye ne-Tip60 kunye ne-ribonucleotide reductase.Uhlalutyo lwethu lubonise ukuba i-N-terminal 32-amino-acid ye-CMPK iyadingeka ekuqeshweni kwayo kwiindawo zomonakalo we-DNA ngendlela exhomekeke kwi-Tip60.Ukuphinda kuvezwe kohlobo lwasendle kodwa hayi i-N-terminus ecinyiweyo i-CMPK ibuyisela ukusebenza kakuhle kokulungiswa kwe-DNA kwiiseli ze-CMPK zokuwisa.Sicebise ukuba ukubunjwa kwe-dCDP yesayithi ethile nge-CMPK kubonelela ngeendlela zokuququzelela ukulungiswa kwe-DNA kwiiseli ze-serum-deprived.
