Deoxyribonucleic acids,isidoda sentlanzi Cas:100403-24-5
| Inombolo Yekhathalogu | XD90579 |
| Igama lemveliso | Deoxyribonucleic acids, idlozi lentlanzi |
| CAS | 100403-24-5 |
| Ifomula yeemolekyuli | - |
| Ubunzima beMolekyuli | - |
| Iinkcukacha zokuGcina | 2 ukuya ku-8 °C |
| IKhowudi yoMrhumo eHarmonized | 29349990 |
Ukucaciswa kweMveliso
| Imbonakalo | Umgubo omhlophe |
| Isivavanyi | 99% |
Iindlela ze-molecular pathogenic kwizifo zesibindi ezifana ne-hepatitis C (HCV), i-autoimmune hepatitis (AIH) kunye ne-primary biliary cirrhosis (PBC) ibonakaliswe kakubi.Iimfuza ezichazwe ngokwahlukileyo zihlala zibalulekile kwi-pathogenesis yesifo.I-Sppression subtractive hybridization (SSH) yindlela ebanzi ye-genome etyebisa ushicilelo oluchazwe ngokwahlukileyo lwe-mRNA.Sijonge ukwenza uqwalaselo lwenoveli yolwahlulo lwembonakalo yemfuza kwi-cirrhosis kusetyenziswa i-SSH edityaniswe nenani lexesha lokwenyani le-reverse transcriptase polymerase chain reaction (RT-PCR).I-transcriptomes yesibindi kwi-HCV cirrhosis, i-cirrhosis ye-AIH, i-PBC, kunye nezicubu zesibindi ezingekho phantsi zihlolwe yi-SSH.Iziphumo ezihambelanayo ze-DNA (cDNA) iiclones zaphinda zavavanywa ukuze zibonakaliswe ngokwahlukileyo ngokuxutywa kwe-dot-blot emva koko zilandelelaniswe.Ukubonakaliswa kofuzo okukhethiweyo kulinganiswe ngexesha lokwenyani i-RT-PCR.Ukulandela i-SSH, ii-clones ezingama-694 ziye zaphinda zavavanywa ukuze zibonise umahluko wemfuza, apho i-145 ilandelelaniswe kwaye yafunyaniswa ukuba iphuma kwi-89 yemfuza eyahlukeneyo.Ii-clones ezisixhenxe zazine-homologous kuphela ngethegi echaziweyo yolandelelwano (EST) ngolandelelwano lwe-encoding genes engenamsebenzi waziwayo.Ukubonakaliswa okuphezulu kwezakhi zofuzo ezine kwaqinisekiswa yi-RT-PCR yexesha langempela: i-transmembrane 4 ilungu le-superfamily 3 (tetraspanin CO-029) kuzo zonke iintlobo ze-cirrhosis, i-hedgehog esebenzisana neprotheni (HIP) kwi-AIH cirrhosis kunye ne-chitinase 3-like-1 (HC gp-39 okanye ykl-40) kunye ne-arginine-glutamic acid iphinda (RERE) kwi-HCV cirrhosis.I-RERE i-polymorphisms yemfuza kunye nokwahluka kwe-splice kwabonwa kuzo zonke izicubu ezihlolwe.I-Tetraspanin CO-029 up-regulation yayilungiselelwe indawo kwiiseli ze-bile ductular.Ukuqukumbela, uqwalaselo lwenoveli lokubonakaliswa kofuzo olwahlukileyo kwi-cirrhosis yomntu lwenziwa kusetyenziswa i-SSH njengesixhobo sokuqala sokufumanisa.Ngokukodwa, uphando olongezelelweyo lwejini ye-RERE kunye neemveliso zayo kwi-HCV ehambelana nesifo sesibindi zifanelekile.
