Iimveliso

Uvavanyo olulula lwamabala luye lwaphuhliswa, oluvumela ukubalwa kwe-microalbuminuria.Uvavanyo lwenziwe ngokwezikhokelo ze-ISO 15189. Umchamo wabonwa kwimicu ye-cellulose acetate kwaye ungcoliswe kusetyenziswa iidayi ezibophelelayo zeprotheyini ezahlukeneyo (i-nigrosin, i-Coomassie Blue R-250, i-amido emnyama).Ubunzulu bombala wamabala angcoliswe buqikelelwa kusetyenziswa isitishi se-Kodak Image 450. Uvakalelo lohlalutyo lwendlela esekelwe kwi-Coomassie Blue (18 mg/L) yayingcono kune-nigrosin (50 mg/ L) okanye i-amido emnyama (100 mg/L) ) iindlela ezisekelwe.I-coefficient yangaphakathi yokwahlukana (i-CV) kunye ne-CV ephakathi kovavanyo lwe-Coomassie blue assay ibe, ngokulandelelanayo, yi-8.4% kunye ne-9.7% (50 mg/L), kunye ne-3% kunye ne-4.5% (400 mg/L).Kwi-nigrosin, ezi datha, ngokulandelanayo, i-8.4 kunye ne-9.4 (50 mg / L), kunye ne-3.4 kunye ne-6.4% (400 mg / L).I-Coomassie Blue ibonise ukhetho olukhethekileyo olubophelelayo kwi-albumin.Indlela yafunyaniswa ukuba inomgca phakathi kwe-20 kunye ne-600 mg / L.Ulungelelwaniso olulungileyo (r2 = 0.89) lufunyenwe phakathi kweCoomassie Blue esekwe kunye nemilinganiselo ye-immunonephelometric.I-albumin ye-Immuno-unreactive albumin (elungiswe lunyango lwe-protease) inokubonwa ngovavanyo lwamabala, olunika inzuzo yendlela xa kuthelekiswa novavanyo lwe-immunochemical.Imvula ye-ammonium sulphate inokunyusa ngakumbi ukuchana kovavanyo ngokususa iziphumo zamatyathanga okukhanya asimahla. Le ndlela ichaziweyo ilula kakhulu kwaye inexabiso eliphantsi kakhulu, nto leyo eyenza ukuba ifanelekele iinkqubo zokuhlola, ngakumbi kumazwe ehlabathi lesithathu.